用HPLC法测定国内反应香精中的4,8-DiMeIQx和PhIP  

Determination of 4,8-DiMeIQx and PhIP in Domestic Process Flavor by HPLC

作　　者：崔俭杰[1,2] 李琼[1,2] 金其璋 

机构地区：[1]上海应用技术学院,上海200235 [2]上海香料研究所,上海200232

出　　处：《化学世界》2010年第2期87-90,86,共5页Chemical World

基　　金：上海市教委基金项目资助(05D205009)

摘　　要：建立同时测定反应香精中2-胺基-3,4,8-三甲基-3H-咪唑并[4,5-F]喹喔啉(4,8-DiMeIQx)和2-胺基-1-甲基-6-苯基咪唑[4,5-b]嘧啶(PhIP)的高效液相色谱(HPLC)分析方法。用强酸水解反应香精,在碱性环境下用二氯甲烷萃取分析物,弱酸净化。以乙腈和pH3.44的醋酸-醋酸胺缓冲溶液梯度洗脱,264 nm(测定4,8-DiMeIQx)和312 nm(测定PhIP)双波长扫描,进行高效液相色谱检测。4,8-DiMeIQx和PhIP在反应香精中的检出限均为0.01 mg/L;加标回收率分别为87.25%和102%。用该法对国内不同公司的不同产品进行检验适用性良好。A high-performance liquid chromatography （HPLC） analytical method for,determination of 2- amino-l-methyl-：6-phenylimidazo [-4,5-b] pyridine （PhIP） and 2-amino-3,4,8-trimethyl-3H-imidazo [-4,5-F] quino xaline （4, 8-DiMeIQx） in process flavor was estalliohed. The process fla,;or samples were hydrolyzed by a strong acid. The sample was extracted from the solution after treatment by dichloromethane in alkaline environment, and then purified by a weak acid. Acetonitrile and pH3.44 acetic acid-ammonium acetate buffer solution were used as the mobile phase, and 264 nm （determining 4, 8- DiMeIQx） and 312nm （determining PhIP） were the detective wavelengths. The detective limitation values for the two heterocyclic amines （HCAs） in process flavor were both 0. 01 mg/L, and the recovery was 87.25% for 4, 8-DiMeIQx and 102 % for PhiP. This technique was usable for the determination of process flavor products supplied from different companies.

关 键 词：反应香精 杂环胺 4 8-DiMeIQx PHIP 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]