气相色谱-质谱法测定奶粉中的环境雌激素  

DETERMINATION OF ENVIRONMENTAL ESTROGENS IN MILK POWERDER BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

作　　者：张宏[1] 王淑惠[1] 杨立学[1] 王婷婷[2] 张红英[1] 陈杏琴[1] 

机构地区：[1]石家庄市疾病预防控制中心,050011 [2]河北师范大学实习生

出　　处：《现代预防医学》2010年第4期636-638,640,共4页Modern Preventive Medicine

基　　金：石家庄市科技局指令性课题(04146403A)

摘　　要：[目的]建立奶粉中的环境雌激素(4-壬基酚、双酚A、已烯雌酚、17α-乙炔基雌二醇)和内源性雌激素(17α-雌二醇、17β-雌二醇、雌三醇、雌酮)的气相色谱-质谱(GC-MS)测定方法。[方法]样品经乙腈沉淀蛋白质后取上清液氮气流浓缩至干,经五氟丙酸酐(PFPA)衍生化后用GC-MS进行检测。[结果]该法测定的峰面积的日内相对标准偏差为1.25%～5.85%,检出限为0.024～1.40μg/kg。回收率为72.2%～102.5%。[结论]该方法准确、灵敏,精密度好,适于奶粉中环境雌激素的测定。[Objective] To establish the gas chromatography--mass spectrometricmethod （GC/MS） for simultaneous determination of environmental estrogens including g.nonylphenol, bishphenol A, diethylstilbestrol and 17α-ethinylestradiol and endogenic estrogens including 17α-estradiol, 17β-estradiol, estriol and estrone in milk powder. [Methods] Acetonitrile was used as extractant and protein precipitator. The extract was evaporated to dryness with nitrogen and then derivated with pentafluoropropionic anhydride （PFPA）, the derivatives were determined by GC-MS. [ Results] The intra-day relative standard deviations of the peak area were 1.25% to 5.85%, the detection limits ranged from 0.05 to 1.30μg/kg and the recoveries ranged from 72.2% to 102.5%. [Conclusion] This method is simple, rapid, accurate and applicable to the determination of trace environmental estrogens in milk powder.

关 键 词：气相色谱-质谱法 环境雌激素 奶粉 

分 类 号：R155.5[医药卫生—营养与食品卫生学]