多烯紫杉醇对人胆管癌细胞和胰腺癌细胞增殖和凋亡的影响  

The effect of docetaxel on the proliferation and apoptosis of human cholangiocarcinoma cell line and pancreatic cancer cell line

作　　者：尚鸣异[1] 张岩[1] 高晓龙[1] 唐俊军[1] 席芊[1] 黄宗良[1] 胡美玉[2] 王培军[1] 

机构地区：[1]同济大学附属同济医院放射科,上海200065 [2]复旦大学附属中山医院中心实验室

出　　处：《介入放射学杂志》2010年第1期46-48,共3页Journal of Interventional Radiology

摘　　要：目的评价多烯紫杉醇对人胆管癌细胞HCCC-9810和胰腺癌细胞BXPC-3的增殖和凋亡影响,为药物涂层支架治疗恶性梗阻性黄疸的应用提供实验基础。方法应用四硝基偶氮唑蓝法测定紫杉醇对人胆管癌细胞HCCC-9810和胰腺癌细胞BXPC-3增殖的抑制作用,Hoechst染色法检测诱导细胞凋亡。结果多烯紫杉醇可明显抑制2种肿瘤细胞株的增殖,且该抑制效应随药物剂量的增加和作用时间的延长而增强(HCCC:χ2=24.42,P<0.01;BXPC-3:χ2=24.68,P<0.01),根据增殖曲线和统计学分析,选取0.4mg/L为多烯紫杉醇最适实验浓度。凋亡分析实验显示多烯紫杉醇可诱导2种肿瘤细胞株的凋亡(HCCC:χ2=30.05,P<0.01;BXPC-3:χ2=25.22,P<0.01)。结论多烯紫杉醇有抑制BXPC-3和HCCC-9810的增殖并诱导肿瘤细胞凋亡的作用。Objective To assess the effect of docetaxel on the proliferation and apoptosis of human cholangiocarcinoma cell line HCCC-9810 and pancreatic cancer cell line BXPC-3, in order to provide the experimental basis for the clinical treatment of malignant obstructed jaundice with covered stents. Methods MTT assay and Hoechst staining were used to examine the growth inhibition and apoptosis effect on the two cell lines induced by docetaxel. Results Docetaxel could markedly inhibit the proliferation of the two cell lines and the inhibition effect was strengthened with time and dosage （HCCC：X^2 = 24.42, P 〈 0.01, BXPC- 3： X^22 = 24.68, P 〈 0.01）. Apoptosis assay showed that docetaxel could induce the cell apoptosis for both HCCC-9810 and BXPC-3 （HCCC： X^22 = 30.05, P 〈 0.01; BXPC-3：X^22 = 25.22, P 〈 0.01）. Conclusion Docetaxel can inhibit cell proliferation and induce apoptosis in human cholangiocarcinoma cell line HCCC- 9810 and pancreatic cancer cell line BXPC-3.

关 键 词：胰腺癌 胆管细胞癌 多烯紫杉醇 增殖 凋亡 

分 类 号：R735[医药卫生—肿瘤]