脑缺血再灌注大鼠不同时刻乳酸和磷酸肌酸/肌酸的波谱变化  

The variation of magnetic resonance spectrum of lactate and phosphocreatine/creatine after cerebral ischemia/reperfusion in rats at different times

作　　者：闻万顺[1,2,3] 廖维靖[1,2] 叶祥明[3] 杨万同[1,2] 毕博[1,2] 魏妮[4] 张国栋[1,2] 江城[1,2] 

机构地区：[1]武汉大学中南医院康复医学科 [2]武汉大学脑血管病研究中心,湖北武汉430071 [3]浙江省人民医院康复医学科 [4]中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室

出　　处：《中国康复医学杂志》2010年第2期119-122,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(30370357)

摘　　要：目的:研究大鼠脑缺血再灌注后不同时刻乳酸(Lac)和磷酸肌酸/肌酸(PCr/Cr)的波谱变化规律。方法:雄性Wistar大鼠32只,体重160—200g,随机分为正常对照组和实验组。实验组包括大脑中动脉阻塞(MCAO)模型组、假手术组、单纯梗死组,每组8只。采用1H质子波谱分析,各组均作磁共振波谱(MRS)的连续观测,观察Lac和PCr/Cr波峰的改变,以积分面积表示峰域并进行比较。结果:与正常对照组和假手术组比较,MCAO模型组缺血后出现Lac,再灌注后短暂下降继而呈上升趋势;单纯梗死组缺血出现Lac,一段时间维持后再次呈上升趋势。MCAO模型组缺血后PCr/Cr显著下降,再灌注后再次显著下降,低水平维持;单纯梗死组缺血后PCr/Cr显著下降,并在此低水平一直维持。结论:脑缺血再灌注大鼠不同时刻Lac和PCr/Cr的波谱变化可较好地反映缺血后脑组织的能量代谢变化,有无再灌注均不能改变能量缺失变化。Objective：To study the regulation of magnetic resonance spectrum （MRS） of lactate （Lac） and phosphocreatine/creatine（PCr/Cr） after cerebral ischemia/reperfusion in rats at different times.Method：Thirty-two male Wistar rats,weighting 160—200g,were randomly divided into normal control group and operation group,operation group included middle cerebral artery obstruction （MCAO） group,sham-operation group,and simple-infarction group,8 rats in each every group.Proton magnetic resonance spectroscopy was used to analysis areas under the waves of Lac and PCr/Cr and compared the data of integral areas under the waves.All the groups were under continuous observation of MRS.Result：Compared with normal control group and sham-operation group,in MCAO group the wave of Lac appeared after ischemia,descended temporarily after reperfusion and then kept the trend of rising;in simple-infarction group the wave of Lac appeared after ischemia,maintain the level at sometime and then changed the trend of rising.In MCAO group the wave of PCr/Cr descended significantly after ischemia,then descended significantly again after reperfusion,maintain the low level.In simple-infarction group the wave of PCr/Cr descended significantly after ischemia,and maintain the low level all the time.Conclusion：The variation of MRS of Lac and PCr/Cr after cerebral ischemia/reperfusion in rats can reflect the energy metabolitsm in brain tissue well.Whether reperfusion or not can not change the deficiency of energy.

关 键 词：脑缺血再灌注 乳酸 磷酸肌酸/肌酸 磁共振波谱 

分 类 号：R743.3[医药卫生—神经病学与精神病学]