局部应用辛伐他汀对拔牙窝内VEGFmRNA表达的影响及意义  

Effects of Simvastatin on VEGF mRNA Expression of the Alveolar Bone following Tooth Extraction

作　　者：方鸿满 刘畅[2] 吴哲[3] 许莹莹[3] 赵磊[3] 孙宏晨[4] 

机构地区：[1]武警吉林省总队医院口腔科吉林长春130052 [2]吉林大学口腔医院正畸科 [3]吉林大学口腔医院修复科 [4]吉林大学口腔医院病理科

出　　处：《口腔医学研究》2010年第1期4-6,共3页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:30872912)

摘　　要：目的:探讨局部应用辛伐他汀促进拔牙术后牙槽骨修复机制。方法:选用健康雄性Wistar大鼠56只,随机分为实验组28只和对照组28只,拔除右下颌中切牙后,实验组即刻植入载辛伐他汀PLGA支架材料,对照组植入单纯PLGA作为对照,于术后5d、1周、2周和4周分别处死大鼠,设计针对VEGF mRNA的寡核苷酸探针,采用原位杂交技术检测拔牙窝VEGF mRNA的表达。结果:术后1周和2周实验组VEGF阳性细胞数和染色强度均高于对照组,且差异有统计学意义,而术后4周,实验组和对照组阳性细胞数无统计学差异,且阳性表达细胞少于术后2周。结论:局部应用辛伐他汀通过增加VEGF mRNA的表达促进拔牙窝骨损伤的愈合。Objective： To investigate the mechanism of topically used Simvastatin on promoting alveolar bone repair following tooth extraction. Methods： Fifty eight male Wistar rats were divided into experimental groups and control groups （28 rats/group）. PLGA scaffold was immediately implanted with or without Simvastatin in extraction sockets of the mandibular incisors. Hybridization technique was conducted after 5days, lweeks, 2weeks and 4weeks. Results： The number of VEGF positive vascular endothelial cells in experimental group was significantly higher than that in control group after 1 and 2 weeks of implantation（P〈0.05）. After 4 weeks of implantation, the number of VEGF--positive cells in experimental group was not significantly higher than that of control group （P〈 0.05）. Conclusion： Simvastain promotes alveolar bone repair following tooth extractiontby up--regulation of VEGF by mRNA expression.

关 键 词：辛伐他汀 牙槽骨VEGF MRNA 

分 类 号：R782.2[医药卫生—口腔医学]