纳滤在L-鸟氨酸后提取中的应用研究  

Study on refinement of L-ornithine by nanofiltration

作　　者：万红贵[1] 朱明新[1] 吴启赐[1] 袁建锋[1] 宗素艳[1] 徐传学[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《膜科学与技术》2010年第1期82-85,共4页Membrane Science and Technology

摘　　要：研究了采用纳滤技术代替活性碳对L-鸟氨酸发酵液进行脱色的可行性.从6种有机膜中选择了截留分子量为500的纳滤膜,并考察了料液pH、温度、压力、离子强度对L-鸟氨酸膜透过性能的影响.结果表明膜透过最优条件为:料液温度t=45℃、pH=9.0、操作压力p=2.0 MPa,在此条件下渗滤透过液的透光率T430 nm=96%,L-鸟氨酸收率达到95.8%.此方法在技术上是完全可行的.The feasibility of applying the nanofiltration （NF） instead of Activated carbon in the decolorization of L- ornithine fermentation broth was studied. The NF membrane with MWCO 500 from six organic mem- branes. The permeation behavior of L - ornithine in broth were investigated under different pH value, tempera- ture, pressure and ionic strength. The results show that the optimal conditions of permeation are t = 45 ℃, pH = 9.0, p = 2.0 MPa. In the optimized operating conditions, the transmittance of diafiltration permeation solution at 430 nm is 96 %, the yield of L - ornithine reached 95.8 %. The results also shows that the method adoptedin this work is technically feasible.

关 键 词：纳滤 L-鸟氨酸 脱色 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]