黑曲霉固态发酵苹果渣产β-甘露聚糖酶的工艺优化  

Optimization of Solid-state Fermentation Conditions for Production of β-mannanase with Apple Pomace by Aspergillus niger

作　　者：庄童琳[1,2] 郭凤霞[1] 刘成[2] 朱彤[2] 孙中涛[2] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]山东农业大学生命科学学院,山东泰安271018

出　　处：《生物技术》2010年第1期69-73,共5页Biotechnology

基　　金：山东省科技攻关计划项目("山东省优势农产品深加工关键技术及装备研究开发;"No.2005GG1109002)资助

摘　　要：目的:对黑曲霉SL-08固态发酵苹果渣生产β-甘露聚糖酶的生产工艺进行优化,旨在探寻苹果渣的综合利用方式,降低β-甘露聚糖酶的生产成本。方法:采用Plackett-Burman试验设计和响应面法进行优化。结果:最佳培养基组成为苹果渣与棉粕1∶1(w/w)、尿素2%(w/w)、KH2PO40.1%(w/w)、初始含水率59%(w/w)、CaCl20.2%(w/w)、MgCl20.1%(w/w),30℃恒温培养48h,β-甘露聚糖酶酶活力可达539U/g干曲,比基础培养基提高了28.3%,达到了以豆粕与麸皮为生产原料时的产酶水平。结论:采用黑曲霉SL-08对苹果渣进行固态发酵是一种有效的生物转化方式,既可用于β-甘露聚糖酶的生产,取代豆粕与麸皮等常规原料,降低生产成本;也可以对苹果渣进行综合利用。Objective： In order to reduce the production cost of β - mannanase and make the best of apple pomace, the fermentation conditions for Aspergillus niger SL - 08 to produce β - mannanase with apple pomace as raw material in solid - state fermentation were optimized. Method： Plackett -Burman design and response surface methodology were adopted to optimize the fermentation medium. Result： The mixture of apple pomace and cottonseed powder （1： 1, w/w） with 59% （w/w）initial moisture, supplemented with 2% （w/w） urea, 0.1% （w/w） KH2 PO4, 0.2% （w/w） CaCl2 and 0.1% （w/w） MgCl2, was proved to be the optimmn medium. When the test fungi were inoculated in the optimized medium and incubated at 30℃ for 48h, the activity of β - mannanase increased by 28.3% than that in the basal medium and achieved 539 u/g, which was as high as the enzyme activity obtained when the bran was used as the raw material. Conclusion： Solid -state fermentation with apple pomace as raw material by Aspergillus niger SL -08 was proved to be an effective way for both the production of β - mannanase and the comprehensive utilization of apple pomace.

关 键 词：苹果渣 Β-甘露聚糖酶 固态发酵 响应面分析 

分 类 号：Q939.5[生物学—微生物学]