高效液相色谱法测定蜜饯中的甘素  

Determination of dulcin in succade by HPLC

作　　者：杨雪娇[1] 付登洲[1] 黄伟[1] 吕维佳[1] 

机构地区：[1]东莞出入境检验检疫局,东莞523072

出　　处：《中国食品添加剂》2010年第1期210-213,共4页China Food Additives

摘　　要：目的：建立蜜饯食品中甘素的测定方法。方法：样品加水溶解后,沉淀蛋白质,取上清液用磁力搅拌器提取后过滤用高效液相色谱测定。用SB-C18色谱柱（4.6mm×150mm,5μm）作固定相,以磷酸二氢钾溶液-乙腈（60＋40）为流动相,在240nm波长的条件下采用二极管阵列检测器进行检测。结果：线性范围为1.0-200.0μg/mL,方法的测定下限（S/N=3）为0.2μg/mL,加标回收率范围在88.3%-94.0%之间,相对标准偏差在0.0772-2.65之间。Aim：To establish the method of determination of dulcin in succade by HPLC.Method：After the sample was dissolved in water and protein was deposit,the clear liquid obtained was extracted by magnetic beater,filtrated and detected by HPLC.The chromatographic column of SB-C18（4.6mm×150mm,5μm）was used as stationary phase,and Potassium dihydrogen phosphate（KH2PO4）solution-Acetonitrile（60＋40）was used as mobile phase.UV-detection at 240nm with DAD was adopted in the determinations.Result： The range of linear relationship was1.0~200.0μg/mL,The detection limits（S/N=3）was 0.2μg/mL,The range of recovery was 88.3%~94.0%,and the range of RSD was 0.0772~2.65.

关 键 词：甘素 液相色谱法 蜜饯 

分 类 号：TS207.3[轻工技术与工程—食品科学]