Folin-Ciocalteu比色法测定丝瓜中多酚含量的研究  

Studies on Polyphenol Content Determination of Luffa by Folin-Ciocalteu Colorimetry

作　　者：黄树苹[1] 谈太明[1] 徐长城[1] 谈杰[1] 刘明[1] 王萌[2] 

机构地区：[1]武汉市蔬菜科学研究所,湖北武汉450065 [2]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《中国蔬菜》2010年第4期47-52,共6页China Vegetables

基　　金：武汉市农业科学技术研究院院立科技项目

摘　　要：以没食子酸为标准品,采用Folin-Ciocalteu比色法研究了丝瓜中多酚的定量测定方法。结果表明,2mL的丝瓜多酚提取液加入Folin-Ciocalteu试剂2mL,10%的Na2CO34mL,在30℃的条件下反应90min,测定其在770nm处的吸光值,多酚质量浓度在2～10mg.L-1范围内与吸光值呈良好的线性关系,根据此线性回归方程进行丝瓜多酚的定量测定,稳定性强,重现性好,精密度高。A quantitative determination method for polyphenol content in luffa was studied by using Folin-Ciocalteu colorimetry with gallic acid as standard.The results showed that 2 mL polyphenol contents from luffa could be well calculated according to their colorimetric absorption at 770 nm by applying 2 mL Folin-Ciocalteu reagent,and 4 mL 10 % Na2CO3 for 90 min at 30 ℃ for colorimetric reaction,and the linear range of standard curve was 2-10 mg·L-1.The polyphenol content in luffa could be determined by this method.This method is very stable with good reappearing property and high accuracy.

关 键 词：丝瓜 多酚 Folin-Ciocalteu比色法 

分 类 号：S642.4[农业科学—蔬菜学]