固定化菱形藻(Nitzschia sp.)的生长及营养成分变化  

Growth and nutritional ingredient of immobilized marine benthic diatom,Nitzschia sp.

作　　者：邢荣莲[1] 苏杭[1] 苏群[1] 王长海[1] 

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《中国水产科学》2010年第2期274-280,共7页Journal of Fishery Sciences of China

基　　金：国家科技支撑计划(2008BAD94B04);山东省优秀中青年科学家科研奖励基金(2008BS06012);山东省黄河三角洲生态环境重点实验室开放基金资助项目(2009KFJJ02)

摘　　要：选用底栖硅藻菱形藻(Nitzschiasp.)为实验对象,分别研究了CaCl2浓度、胶珠直径、接种密度及胶珠密度对其生长的影响及后两因素对其主要营养成分含量变化的影响。结果表明:(1)随培养时间的延长,CaCl2浓度对菱形藻生长有极显著影响,在2%CaCl2溶液中形成的胶珠里菱形藻比生长速率最高;(2)胶珠直径对菱形藻在初期和指数期的生长影响显著,且较小直径(2.5～3.0mm)的胶珠较适宜菱形藻生长;(3)随接种密度和胶珠密度的提高,菱形藻的比生长速率都有先增高后下降的变化趋势,接种密度较高(2.50×104～5.50×104mL-1)时,固定化组的比生长速率是对照组的2倍左右。胶珠密度为220粒/(100mL培养液)左右较适宜(胶珠直径3.0mm);(4)初始接种密度和胶珠密度试验中,蛋白质、总糖及粗脂肪的含量固定化组低于或相当于对照组,而叶绿素的含量固定化组高于对照组。The growth and nutritional ingredient of Nitzschia sp., a marine benthic diatom, in alginate beads were studied. The main factors during the immobilized culture including CaCl2 concentration, the diameter of alginate bead, initial inoculum density and the density of alginate bead were investigated. The results indicated that： （ 1 ）With the culture days increasing, the specific growth rates were significantly affected by CaCl2 concentration. The specific growth rate of Nitzschia sp. in beads with 2% CaCl2 was the highest. （ 2 ） The diameter of alginate bead remarkably influenced the growth of benthic diatom in stationary phase and exponential phase. The suitable diameter of alginate beads for cell growth were 2.5-3.0 mm. （ 3 ） There was a tendency of increasing at first and then decreasing in growth rate of benthic diatom with the increasing of inoculum density and number of alginate beads. The specific growth rate of benthic diatom in the immobilization groups was about 2 times as much as that in the control groups as the inoculum densities were 2.50 × 10^4-5.50 × 10^4 mL^-1. The optimal density of alginate beads was 220 pills/100 mL. （ 4 ） In the experiment of initial inoculnm density and the density of alginate bead, the contents of protein, total carbohydrate and crude fat in the control groups were more than that in the immobilization groups. The content result of chlorophyll compared with above result was opposite. [Journal of Fishery Sciences ofChina,2010, 17 （ 2 ） ： 274-280]

关 键 词：底栖硅藻 菱形藻 固定化 比生长速率 营养成分 

分 类 号：S97[农业科学—捕捞与储运]