金属离子对枯草芽孢杆菌BS501a发酵液防治稻瘟病菌效果的影响  

EFFECT OF METAL IONS ON THE ANTAGONISTIC EFFECT OF BACILLUS SUBTILIS STRAIN BS501a ON MAGNAPORTHE GRISEA

作　　者：李瑞芳[1] 徐怡[1] 田泱源[1] 张长付[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2010年第1期80-83,共4页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南省高等学校青年骨干教师资助计划项目(2008051);郑州市科技攻关计划项目(7-16)

摘　　要：为提高枯草芽孢杆菌拮抗性次生代谢产物的产量，通过单因素试验研究了CaCl2、FeSO4、MnSO4、MgSO4、ZnSO4等金属无机盐对枯草芽孢杆菌BS501a发酵液拮抗效果的影响．结果表明：当分别向基础培养基中添加0．15％-0．2％CaCl2·2H2O、0．01％FeSO4·7H2O、0．01％MnSO4·H2O、0．05％MgSO4·7H20和0．1％ZnSO4·7H2O时，发酵液抗稻瘟病菌活性均有不同程度提高．通过正交试验，确定各种金属无机盐的最佳组合为：0．15％CaCl2·2H2O、0．01％FeS04·7H2O、0．01％MnSO4·H2O、0．05％MgSO4·7H2O和0．15％ZnSO4·7H2O．枯草芽孢杆菌BS501a用优化后的合成培养基发酵，发酵上清过滤液抑菌圈直径达到71．4mm．利用枯草芽孢杆菌BS501a发酵上清过滤液室内防治稻瘟病，防治效率为63．86％．In order to improve the output of antagonistic secondary metabolites of Bacillus subtilis, the effects of metal inorganic salts, such as CaCl2 , FeSO4 , MnSO4, MgSO4 and ZnSO4, on the antagonistic effect of the fermentation supernatant of Bacillus subtilis strain BS501 a were studied by single factor experiment. The results show that the antagonistic effects of the fermentation supernatant on Magnaporthe grisea are enhanced by differ- ent degrees when 0.15% to 0.2% CaCl2· 2H2O, 0.01% FeSO4 · 7H2O, 0.01% MnSO4 · H2O, 0.05% MgSO4 · 7H2O and 0.1% ZnSO4 · 7H2O were added in the basic medium respectively. The orthogonal exper- iment shows that the optimum concentration of metal inorganic salts in the compound medium is as follows： 0.15% CaCl2 ·2H2O, 0.01% FeSO4·7H2O, 0.01% MnSO4· H2O, 0.05% MgSO4·7H2O and0.15% ZnSO4 · 7H2O. Bacillus subtilis strain BS5Ola was fermented in the optimum compound medium, and the di- ameter of the inhibition zone of the filtrate of the fermentation supernatant was up to 71.4 ram. In greenhouse experiments, the filtrate of the fermentation supernatant of the strain BSS01 a was effective in controlling rice blast, and the suppression efficiency was up to 63.86%.

关 键 词：枯草芽孢杆菌 拮抗活性 培养基优化 正交试验 金属离子 

分 类 号：TS210[轻工技术与工程—粮食、油脂及植物蛋白工程]