基于角质酶反胶束预处理的羊毛织物蛋白酶加工  

Enzymatic processing for wool fabric based on cutinase pre-treatment in reverse micelles followed by protease treatment in aqueous phase

作　　者：王平[1] 范雪荣[1] 马小云[1] 王强[1] 崔莉[1] 赵先飞[1] 

机构地区：[1]生态纺织教育部重点实验室(江南大学),江苏无锡214122

出　　处：《纺织学报》2010年第3期78-82,共5页Journal of Textile Research

基　　金：国家高科技研究发展计划项目(2008AA02Z203);常州市新型材料重点实验室开放课题(200801)

摘　　要：为提高羊毛表面类脂物去除效果,增加纤维表面亲水性和后续水相条件下Savinase蛋白酶酶解效率,考察了反胶束体系中表面活性剂、角质酶用量等因素对羊毛织物润湿性、减量率与碱溶解度的影响。研究结果表明,以环己烷、正丁醇和Tween-80制备的反胶束体系中,当三者体积比为5∶1∶1,水与Tween-80物质的量比为25,角质酶用量为2%(o.w.f)时,羊毛织物经蛋白酶处理后具有较好的润湿性能,纤维损伤较少。氨基酸分析结果表明,与仅经蛋白酶处理试样相比,经角质酶、蛋白酶处理后羊毛纤维中胱氨酸相对质量浓度下降。SEM观察验证了经角质酶反胶束体系预处理、蛋白酶处理后,羊毛纤维表面鳞片层得到有效去除。To improve the wettability of wool surface by lipid removal and the efficiency of succeeding proteolytic reaction by Savinase in aqueous phase,the effects of surfactant,cutinase concentrations and others in reverse micelles on wettability,weight loss and alkaline solubility of wool fabrics were studied.The results indicated that the protease-treated wool fabrics presented desirable wettability and acceptable fiber damages when pretreated with 2%（o.w.f） of cutinase in the reverse micelles（the volume ratio of cyclohexane,alcohol and Tween-80 was 5∶1∶1,and the molar ratio of H_2O and Tween-80 was 25）.The result from amino acid analysis showed that the relative mass concentration of cystine decreased remarkedly after the combination of cutinase and protease treatments.SEM images further proved that the combined enzymatic process led to a more satisfactory removal of scales from wool surface.

关 键 词：角质酶 反胶束 SAVINASE蛋白酶 水相 羊毛 润湿性 

分 类 号：TS195.5[轻工技术与工程—纺织化学与染整工程]