产朊假丝酵母生长条件的优化  

Candida utlis growth optimization

作　　者：何东东[1] 张坤生[1] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134

出　　处：《食品科技》2010年第2期14-17,共4页Food Science and Technology

摘　　要：通过安瓿管复壮的产朊假丝酵母,用YPD培养基进行生长曲线的测定,得出12h是此种酵母菌的生长最优条件。对产朊假丝酵母进行单因素条件筛选,包括培养温度、摇床转速、碳氮比,实验得出温度在30℃、转速180r/min、碳氮比(葡萄糖和蛋白胨的质量比)为3∶1时菌体密度最高。通过采用L9(34)正交实验设计确定各因素对酵母菌生长的影响,优化条件为培养时间13h、温度30℃、转速160r/min、碳氮比约3∶1,此时菌体密度最高。Ampoule tube through the rejuvenation of Candida yeast through the use of YPD medium for the growth curve measured 12 hours is reached such optimal conditions for the growth of yeast. Later on Candida yeast single factor in the selection conditions, including temperature, rotation speed, carbon and nitrogen ratio, temperature tests come in 30 ℃, speed 180 r/min, carbon and nitrogen ratio （glucose and peptone than the quality） to 3：1 at the highest cell density. Through the use of post-L9（34） orthogonal design to determine the factors for the growth of yeast. Optimize the conditions for the final training time 13 h, temperature 30 ℃, speed 160 r/min, carbon and nitrogen ratio of 3∶1 at the highest cell density.

关 键 词：产朊假丝酵母 YPD培养基 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]