纤维素酶用量和底物浓度对玉米秸秆酶解的影响  

Effects of cellulase loading and substrate concentration on the enzymatic hydrolysis of corn stover

作　　者：何珣[1] 缪冶炼[1] 陈介余 蒋学剑[1] 徐子栋[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学食品与轻工学院,材料化学工程国家重点实验室,南京210009 [2]日本秋田县立大学生物资源学部

出　　处：《食品科技》2010年第2期47-51,55,共6页Food Science and Technology

基　　金：国家自然科学基金委-广东省政府联合基金(U0733001);国家重点基础研究发展计划(973项目)(2009CB724700)

摘　　要：首先采用碱液湿磨法对玉米秸秆进行预处理,然后对预处理玉米秸秆进行酶解,调查了纤维素酶用量、底物浓度对还原糖收率和反应速度的影响,同时讨论了木质素对纤维素酶解的抑制机理。纤维素酶用量在1.5～30FPU/g的范围内变化,底物浓度在10～40g/L的范围内变化。通过对预处理玉米秸秆酶解的响应面分析,得到了还原糖收率与纤维素酶用量、底物浓度之间的关系式。实验结果表明,纤维素酶用量越大,酶解反应速率随底物浓度的增加幅度也越大。木质素对纤维素酶的吸附会造成纤维素酶的失活,从而导致酶解反应速率和还原糖收率的降低。Corn stover, which was pretreated with an integrated wet-milling and alkali pretreatment method, was enzymatically hydrolyzed by a commercial cellulatic complex under conditions of a cellulase loading in the range of 1.5 ～30 FPU/g and a substrate concentration in the range of 10 ～40 g/L. The effects of cellulase loading and substrate concentration on reducing-sugar yield and hydrolysis reaction rate were investigated, and the mechanism of inhibiting hydrolysis reaction by lignin was discussed. A regression equation of reducing-sugar yield and cellulase loading, substrate concentration was obtained using the response surface methodology. It was shown that at a larger cellulase loading, reaction rate had a larger increase with increasing of substrate concentration. Adsorption of cellulase on lignin caused the inactivation of cellulase, which resulted in the reduction of hydrolysis reaction rate and reducing-sugar yield.

关 键 词：木质纤维素 玉米秸秆 预处理 酶解 响应面分析 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]