用硅橡胶膜生物反应器系统选育高性能酿酒酵母  

Screening of High Fermentability Saccharomyces cerevisiae by PDMS Composite Membrane Bioreactor

作　　者：陈春燕[1] 肖泽仪[1] 汤晓玉[1] 周一慧[1] 蒋越[1] 傅晟伟[1] 

机构地区：[1]四川大学化工学院

出　　处：《食品与发酵工业》2010年第2期8-11,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(20776088)

摘　　要：应用硅橡胶膜生物反应器的发酵-渗透汽化耦合功能,进行长期封闭循环发酵,使酵母在这种特定环境下完成适应性自然进化,从而获得优势的突变株。以工业安琪酵母ADY为原始菌株,进行每一轮500h的封闭循环发酵,在发酵终点取菌悬液做平板筛菌,分离出遗传约200代的相对强势的新菌株,再转移新菌株进行同样的发酵和终点筛选实验。如此的发酵-筛选-转移实验共进行了3轮。试验结果表明:ADY遗传超过600代的菌株S33,在500h封闭循环发酵残液配制的培养基条件下,表现出了明显的环境适应性遗传优势,其细胞生长速率、产物乙醇的比生成速率和糖转化率都相对原种菌ADY分别提高了约71.8%、53.6%和52.7%。A Saccharomyces cerevisiae could be acclimated and evolved by a long continuous and close circulation fermentation in the PDMS membrane bioreactor system which coupled the fermentation with pervaporation, and a superior mutant strain could be obtained. In this work, Angel yeast （ADY） was taken as the parent strain and underwent a continuous and close circulation fermentation of 500 h. At termination of the fermentation, as a descendant of 200 generations of ADY, a preferable strain was screened from the residual broth by a usual method. The screened strain was transferred and inoculated into next fermentation and the same screening was conducted as previous fermentation. The fermentation - screening - transfer procedure was repeated three times until a 600 - generation descendant was screened. The strain S33 from the third run was showing great superiority over ADY under a substrate prepared by the residual broth after 500 h fermentation, with the cell growth rate, ethanol yield rate and glucose conversion percent increased by 71.8% ,53.6% and 52.7% respectively.

关 键 词：硅橡胶膜生物反应器 酿酒酵母 封闭循环发酵 适应性进化 

分 类 号：TS261.1[轻工技术与工程—发酵工程]