高脂日粮介导小鼠十二指肠氧化应激与肠钙吸收的关系  

THE CORRELATION BETWEEN DUODENAL OXIDATIVE STRESS MEDIATED BY HIGH FAT DIET AND INTESTINAL CALCIUM ABSORPTION IN MICE

作　　者：肖瀛[1] 崔珏[1] 施用晖[2,1] 孙进[1] 乐国伟[2,1] 

机构地区：[1]江南大学食品学院,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《营养学报》2010年第1期30-34,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30571347)

摘　　要：目的探讨高脂日粮对小鼠十二指肠氧化还原状态与肠钙吸收的影响及其相关性。方法50只5周龄C57BL/6雄性小鼠,体重13～14g,根据体重随机分为对照组(0.6%Ca,w/w),高脂日粮组(19.64%猪油,0.6%Ca),添加0.1%硫辛酸(LA)高脂日粮组,钙补充高脂日粮组(1.6%Ca)和添加0.1%LA钙补充高脂日粮组,饲养8w,最后1w,通过代谢实验评价钙吸收情况,并检测血和十二指肠的活性氧(ROS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、还原型/氧化型谷胱甘肽(GSH/GSSG)和总抗氧化能力(T-AOC)等氧化应激相关指标。结果高脂日粮导致C57BL/6小鼠十二指肠氧化应激并且减少肠钙吸收。钙净吸收与十二指肠的GSH/GSSG(r=0.801,P<0.01)、T-AOC(r=0.777,P<0.05)呈正相关,而与ROS(r=-0.716,P<0.05)呈负相关。结论高脂日粮导致十二指肠氧化应激是肠钙吸收减少的重要原因。Objective To explore the effect of high-fat diet （HFD） on redox states of duodenum and calcium absorption in mice, and to analyze the relation between them. Method Fifty C57BL/6 male mice were randomly assigned to five groups. The control group consumed an ordinary diet （0.6% Ca, w/w）, and other four groups were fed with HFD （19.64%lard, 0.6% Ca）, HFD plus 0.1% lipoid acid （LA）, HFD with calcium supplement （1.6%Ca） and HFD with 1.6% Ca and 0.1% LA supplement. Calcium apparent absorption was measured by mineral balance study after feeding for 8 w. Plasma and duodenum levels of ROS, SOD, CAT, MDA, GSH/GSSG, and T-AOC were measured to evaluate the antioxidant status. Results HFD induced oxidative stress of duodenum and decrease of intestinal calcium absorption in mice. There were positive correlations between calcium apparent absorption with GSH/GSSG （r=0.801, P〈0.01） and T-AOC of duodenum （r=0.777, P〈0.05） , and negative correlation between calcium apparent absorption and ROS （r=-0.716,P〈0.05） of duodenum in mice. Conclusion Oxidative stress is an important factor for decease of intestinal calcium absorption in mice fed HFD.

关 键 词：高脂日粮 肠钙吸收 氧化应激 C57BL/6小鼠 

分 类 号：R151[医药卫生—营养与食品卫生学]