锯叶棕提取物对RPMI8226细胞凋亡相关蛋白表达的影响  

The Serenoa Repens Extract to the Expression of RPMI8226 Cell Apoptosis-Related Protein Influence

作　　者：车玉琴[1] 张立德[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《实用中医内科杂志》2010年第2期3-5,共3页Journal of Practical Traditional Chinese Internal Medicine

摘　　要：[目的]探讨锯叶棕提取物对RPMI8226细胞凋亡的影响以及对细胞凋亡相关蛋白表达的影响。[方法]①体外培养的RPMI8226细胞加入到不同浓度的锯叶棕提取物(0.5或1.0μL/mL)培养24h,利用流式细胞仪测定位于前G1时期的细胞百分比以检测细胞凋亡;②体外培养的RPMI8226细胞加入到锯叶棕提取物(1.0μL/mL)培养24h,应用Western Blot检测PARP、MCl-1蛋白表达。[结果]①锯叶棕提取物诱导RPMI8226细胞凋亡具有剂量依赖性(P<0.05);②RPMI8226细胞暴露于锯叶棕提取物(1.0μL/mL)培养24h,PARP蛋白水平表达明显增加,但不能调整Mcl-1。[结论]锯叶棕提取物诱导RPMI8226细胞凋亡。[ Objective] Discuss serenoa repens extract to RPMI8226 cells apoptosis influence and the effects on the expression of cell apoptosis-related protein. [ Methods] ①RPMI8226 cells cultured in vitro added to different concentrations of serenoa repens extract （0.5 or 1.0 μL/ mL） cultured for 24 hours, make use of flow eytometry to determine the percentages of cells in pre - G1 phase so as to detect the apoptotic cells l②RPMI8226 ceils cultured in vitro added to serenoa repens extract （1.0 μL/ mL） cultured for 24 hours, using Western Blot to detect the protein expression of PARP, MCl - 1. [ Results ] ①serenoa repens extract induced apoptosis in RPMI8226 ceils in a dose - dependent（ P 〈 0.05 ） ;②RPMI8226 ceils were exposed to serenoa repens extract （1.0μL/mL） cultured for 24 hours, the expression of PARP protein level significantly increased, but can not adjust the Mcl - 1. [ Conclusion ] serenoa repens extract induced apoptosis in RPMI8226 ceils.

关 键 词：RPMI8226细胞 锯叶棕提取物 细胞凋亡 PARP MCl—1 

分 类 号：R-33[医药卫生]