枯草芽孢杆菌TKPG011聚谷氨酸发酵培养基的优化  

Medium Optimization for Poly-γ-glutamic Acid Production by Bacillus subtilis TKPG011

作　　者：刘晓鸥[1] 乔长晟[1] 李睿颖[1] 陈笑[1] 朱健辉[1] 

机构地区：[1]天津市工业微生物重点实验室,工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《现代食品科技》2010年第3期253-255,共3页Modern Food Science and Technology

基　　金：天津市科技支撑重点项目(09ZCKFSH00800);天津滨海新区双百科技特派员科技专项(SB20080039)

摘　　要：本文通过单因素试验和正交实验设计研究了碳源、氮源、前体物L-谷氨酸钠和初始pH对Bacillus subtilis TKPG011生物合成γ-PGA的产量的影响,结果表明优化后的发酵培养基为:葡萄糖50g/L,酵母膏10g/L,L-谷氨酸钠30g/L,初始pH值6.5,K2HPO41.5g/L,MgSO4·7H2O0.5g/L。采用优化培养基能使γ-PGA发酵产量达9.20g/L。The optimal fermentation medium for producing 7-PGA by a mutant strain Bacillus subtilis TKPGO11 was studied by single-factor and oahogonal design theory. With shaking-flask experiments, the optimal fermentation medium consisted of 50 g/L glucose, 10 g/L yeast extract, 30 g/L L-Glutamate, 1.5 g/L K2HPO4 and 0.5 g/L MgSOa.7H20 at pH 6.5. With this medium, the yield of γ-PGA in the shaking-flask could be up to 9.20 g/L.

关 键 词：Γ-PGA 枯草芽胞杆菌 培养基 优化 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]