甘薯中去氢表雄酮的提纯工艺研究  

Study on extraction technology of DHEA from sweet potato with ultrasonic auxiliary

作　　者：杨红花[1] 秦宏伟[1] 

机构地区：[1]泰山学院生物科学与技术系,山东泰安271021

出　　处：《食品工业科技》2010年第3期232-235,共4页Science and Technology of Food Industry

基　　金：泰山学院2007引进人才项目(Y07-2-21)

摘　　要：利用超声波辅助法,对甘薯中的活性成分去氢表雄酮(DHEA)的提纯工艺进行了研究。结果表明,超声波提取DHEA的最佳条件为:60℃水浴下预发酵24h,预发酵液加入鲜甘薯质量2%的浓硫酸,90℃水浴下酸水解24h,水解液经20%的NaOH溶液中和,抽滤、离心去除水分,超声波辅助下石油醚浸提40min,过柱净化,HPLC定性、定量测定。该方法能有效地纯化分析甘薯功能因子DHEA,具有产物纯度高、方法简便、收率高等优点。The purification process of active ingredient dehydroepiandrosterone （DHEA） from sweet potato was studied using ultrasonic auxiliary method.The results showed that the best conditions of DHEA ultrasonic extraction were under 50 degrees for pre-fermentation 24h with additional sulfuric acid 2% fold of the quality of fresh sweet potato in pre-fermentation liquor,90 degrees for acid hydrolysis 24h in water bath, then neutralization in 20% NaOH and filtration,centrifugation to remove the moisture.The product obtained was lixiviated in petroleum ether for 40min using ultrasonic auxiliary method and detected by HPLC after column purification for qualitative and quantitative determination.This method can effectively purify and analyze DHEA extracted from sweet potato with high product purity,simple way and higher yield.

关 键 词：甘薯 超声波辅助法 去氢表雄酮(DHEA) 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]