对甲氧基苯磺酰氯柱前衍生生物胺的高效液相色谱分离  

Separation of biogenic amines by high performance liquid chromatography with 4-methoxy benzenesulphonyl chloride as a precolumn derivatization reagent

作　　者：屠春燕[1] 袁艳娟[1] 吴燕娇[1] 唐美华[1] 韦萍[1] 李寿椿 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009 [2]南京中意化工技术开发研究所,南京210037

出　　处：《分析试验室》2010年第3期96-100,共5页Chinese Journal of Analysis Laboratory

摘　　要：采用对甲氧基苯磺酰氯作为柱前衍生试剂,RP-HPLC为分析模式,建立了一种新的生物胺衍生化方法,并对葡萄酒中生物胺含量进行检测。通过液质联用对产物进行定性,研究并确定了最适衍生化条件:衍生温度50℃,缓冲液pH 9.0,衍生时间15 min。实验建立了7种生物胺的HPLC分离方法:Beckman ODS柱;流动相A为10 mmol/L的NH4Ac溶液(pH 6.37),B相为乙腈;采用梯度洗脱;流速1 mL/min;检测波长240 nm,室温。A novel derivative method for the determination of biogenic amines has been developed by use of 4-methoxy benzenesulphonyl chloride.The derivate production identification was performed by HPLC-MS system.The optimized conditions of derivatization were listed as following： the reaction temperature was 50 ℃;the pH value was 9 and the reaction time was 15 min.The chromatographic conditions were as following： Beckman ODS column（5 μm,4.6×150 mm）,the detection wavelength was 240 nm,the flow rate was 1.0 mL/min;Mobile phase A： 10 mmol/L NH4Ac（pH 6.37）,Mobile phase B： ACN;the gradient protocol was applied.The method has many advantages such as sensitivity,simplicity（pmol）,rapidity and stability,which is suitable for the derivatization of biogenic amines and other substances which have amino group.

关 键 词：对甲氧基苯磺酰氯 衍生化 生物胺 HPLC 

分 类 号：O657.7[理学—分析化学]