温差-超声波复合破壁法对花粉破壁率和黄酮得率的影响  

EFFECTS OF WALL-BREAKING METHOD BY TEMPERATURE DIFFERENCE AND ULTRASONIC FOR WALL-BREAKING RATE AND THE YIELD OF FLAVONOIDS OF POLLEN

作　　者：杨芙莲[1] 党云刚[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2010年第1期58-61,共4页Journal of Shaanxi University of Science & Technology

摘　　要：采用温差破壁结合超声波辅助的复合花粉破壁法对花粉进行破壁，考察了不同的温差破壁因素对花粉破壁率和黄酮得率的影响，并以黄酮得率为指标进行正交试验L9（3^3）优化因素，得到温差破壁的最佳工艺条件为：温差100℃、料液比1：15、冷冻时间24h，此条件下花粉破壁率为61．61％，黄酮得率为3．166％．进一步用超声辅助对花粉进行破壁，使其破壁率达到85％～90％，黄酮得率提高到3．310％．同时，通过试验分析阐述了破壁率和黄酮得率两者的联系．The method of wall-breaking by temperature difference and ultrasonic were used m pollen, investigating the influence of different factors of wall-breaking by temperature difference for wall-breaking rate and the yield of flavonoids in pollen, and experiments was optimized by L9 （33） orthogonal test with the yield of flavonoids as the index, optimum conditions were determined as follows, temperature difference 100 ℃ .ratio of material to liquid 1 ： 15,refrigerant time 24 h, under these conditions, the wall-breaking rate of pollen is 61. 61% ,the yield of flavonoids is 3. 166%. And then carrying out wall-breaking by ultrasonic, the wall-breaking rate reached to 85 %-90 %, the yield of flavonoids is 3.31%. Meanwhile, elaborating the relation between wall-breaking rate and the yield of flavonoids by experiment.

关 键 词：花粉 黄酮 温差 超声波 正交试验 

分 类 号：R284.2[医药卫生—中药学]