亚硫酸氢钠在马铃薯切片过程中防褐变作用机理的研究  

The Antibrowning Mechanism of Sodium Bisulfite on Potato During Slicing

作　　者：王伟[1] 胡泳华[1] 黄浩[1] 杨美花[1] 潘志针[1] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2010年第2期256-259,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金项目(30570408);国家科技支撑计划专项(2007BAD07B06);福建省重点科技项目(2007N0051)

摘　　要：多酚氧化酶(PPO)是蔬果酶促褐变的关键酶,抑制该酶活性是防止蔬果褐变的有效措施.在实验中发现NaHSO3对马铃薯PPO具有显著的抑制作用及在马铃薯切片护色中具有防褐变功能.实验结果表明:马铃薯PPO催化L-多巴形成的褐变物质在475 nm波长处有特征性的吸收峰,并且该吸收峰随着NaHSO3浓度的增加明显降低.NaHSO3的抑制作用表现为不可逆效应,既可以延长酶促反应的迟滞时间,也降低了稳态的酶活力.当NaHSO3浓度增至0.12 mmol/L时,酶促反应的迟滞时间从0延长至56 s,稳态的酶活力下降了35.5%.在马铃薯切片保鲜实验中,对照组在第3天褐变指数达到2,第6天已经完全褐变,而50 mmol/L NaHSO3处理的实验组在第6天褐变指数仅为0.6,有效遏制了马铃薯切片的酶促褐变,实验显示,NaHSO3通过抑制马铃薯PPO活力实现防褐变的效应.Polyphenoloxidase（PPO）is key enzyme of enzymatic browning during vegetables and fruits storage. It is an essential and effective way to inhibit the polyphenol oxidase activity in the fresh-keeping of vegetables and fruits. In this paper, the inhibitory effects on potato PPO and color-preservation effects on potato slice of sodium bisulfite have been studied. The results show that the J inhibition of sodium bisulfite on PPO is an irreversible reaction and that sodium bisulfite can result in prolonging the lag time of the enzyme reaction, and decreaseing the steady-state rate of the enzyme activity. When the concentration of sodium bisulfite reached to 0. 12 mmol/L, the enzyme activity lost about 35.5% and the lag time increased from 0 to 56 s. The potato slice of the control group was half browned after 3 days, and entirely browned after 6 days. While treated by 50 mmol/L of sodium bisulfite, the browning index of the test group was only 0.6, indicating that sodium bisulfite could stop the enzymatic browning of potato slice efficaciously by inhibiting the potato polyphenol oxidase activity.

关 键 词：马铃薯 多酚氧化酶 亚硫酸氢钠 防褐变 

分 类 号：Q356.1[生物学—遗传学]