萝卜红花色苷的高效液相色谱法测定  

High Performance Liquid Chromatography Determination of Pelargonidin-3-O-glucoside in Radish Red

作　　者：游辉 欧阳杰[2] 武彦文 杨寅 汪雨 陈舜琮 

机构地区：[1]国产科学仪器应用示范中心,北京100089 [2]北京林业大学生物科学与技术学院食品科学与工程系,北京100083

出　　处：《分析测试学报》2010年第3期310-312,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(30701107);科技部创新方法工作专项资助项目(2008IM041400;2009IM033200)

摘　　要：建立了高效液相色谱法测定萝bE色素中天竺葵-3—O-葡萄糖苷含量的方法。样品用甲醇溶解，净化后用高效液相色谱分析。结果显示，选择合适的流动相可以获得较好的分离效果。实验采用UltimateXB—C18色谱柱（4．6mm×250mm×5μm），乙腈-5％甲酸溶液（体积比24：76）为流动相，流速1．0mL／min，柱温为25℃，紫外检测波长为525nm，进样量为10μL。实验表明，天竺葵-3-O-葡萄糖苷在0．01～0．10g／L范围内线性关系良好，r=0．999，平均加标回收率为97％～100％，检出限为0．0025g／L。方法灵敏、准确、样品处理简单，适用于萝卜红色素中花色苷含量的测定。A high performance liquid chromatographic （HPLC） method was developed for determination of pelargonidin-3-O-glucoside in Radish Red samples. The sample was dissolved in methanol, and separated by a Ultimate XB-C18（4.6 mm×250mm×5μm） column using acetonitrile -5% formic acid （24 ： 76, by volume） as a mobile phase with flow rate of 1.0 mL/min at column temperature of 25 ~C. The UV detection wavelength was set at 525 nm. A good linearity was obtained in the range of 0. 01 -0. 10 g/L with correlation coefficient of 0. 999. The average spiked recoveries of pel- argonidin-3-O-glucoside was 97% - 100% . The limit of detection for the method was 0. 002 5 g/L. The results indicated that the method was simple, sensitive and accurate, and could be applied in the determination of pelargonidin-3-O-glucoside in Radish Red.

关 键 词：萝卜红 天竺葵-3-O-葡萄糖苷 高效液相色谱 

分 类 号：O657.72[理学—分析化学] O629.13[理学—化学]