高效破乳菌的培养条件优化及破乳效能研究  

Culture condition optimization and the demulsification efficiency of a bacterial strain

作　　者：侯宁[1,2] 李大鹏[1] 杨基先[1] 马放[1] 王金娜[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点试验室,黑龙江哈尔滨150090 [2]东北农业大学资源与环境学院,黑龙江哈尔滨150090

出　　处：《中国环境科学》2010年第3期357-361,共5页China Environmental Science

基　　金：国家"973"项目(2004CB418505);黑龙江省重大课题攻关项目(GA06C202)

摘　　要：从大庆油田受石油污染土壤中分离到1株具有较强破乳能力的莫海威芽孢杆菌(Bacillus mojavensis),其24h破乳率>83%.通过正交试验优化了影响该破乳菌生长及破乳效能的发酵条件;考察了影响其破乳效能的环境因素.结果表明,破乳菌体生长的最佳发酵条件为培养温度25℃、摇床转速160r/min、pH9、接菌量10%、培养时间40h;全培养液破乳的最佳发酵条件为培养温度25℃、摇床转速160r/min、pH5、接菌量6%、培养时间20h;破乳的最佳环境条件为pH5～9,温度30～50℃、全培养液与模型乳状液体积比为1:5.A strain belonging to Bacillus mojavensis, with high capability of de-emulsification was isolated from contaminated oily soil in Daqing oilfield.And for O/W model emulsions, more than 83% efficiency of de-emulsification was obtained for the strain within 24h.According to the orthogonal test, the most favorable incubation conditions for growth and de-emulsification efficiency, as well as the environmental factors influencing de-emulsification efficiency of the strain were investigated.The most favorable fermentative conditions for growth were temperature were at 25℃, 160 r/min,and pH 9.0, inoculating ratio of 10%,culturing time for 40h;and that for de-emulsification occurred when temperature were at 25℃,pH 9.0, and 160 r/min,inoculating ratio of 6%,culturing time for 20h.The optimum environmental conditions for de-emulsification were followings：pH5～9, temperature of 30～50℃, the best proportion of the whole bacteria culture and O/W model emulsions was 1：5.

关 键 词：生物破乳 乳状液 正交试验 培养条件 破乳效能 

分 类 号：X172[环境科学与工程—环境科学]