高效液相色谱法测定棉籽仁中棉酚的含量  

HPLC Determination of Gossypol in Cotton Seed Kernel

作　　者：栾姝[1] 孟磊[2] 孙莲[1] 姚军[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054 [2]新疆医科大学分析测试中心,新疆乌鲁木齐830054

出　　处：《食品科学》2010年第4期198-200,共3页Food Science

基　　金：新疆维吾尔自治区高校冬天计划青年教师科研启动基金项目(XJYDU2007S21)

摘　　要：目的:建立棉籽仁中棉酚含量的高效液相色谱测定方法。方法:采用ODS-C18色谱柱(4.6mm×200mm,5μm),柱温25℃,流动相为甲醇-体积分数1%磷酸溶液(85:15,V/V),流速1.0mL/min,检测波长235nm。结果:棉酚质量浓度在1.9～34.9μg/mL范围内线性关系良好;棉酚的加标回收率为97.8%～100.3%,RSD值为0.4%～1.2%。结论:此法操作简便、快速、灵敏、准确,样品处理简便易行,适于测定棉籽仁中棉酚的含量。A HPLC method was developed for the determination of gossypol in cotton seed kernel.The chromatographic separation was carried out on a ODS-C18 column （4.6 mm × 200 mm,5 μm） with a mobile phase composed of methanol and 1% phosphoric acid aqueous solution （85：15,V/V） at a flow rate of 1.0 mL/min.The detected wavelength was set at 235 nm.Results showed that a good linear relationship between HPLC peak area and gossypol concentration was obtained over the range of 1.9-34.9 μg/mL.Mean spike recoveries of gossypol at three levels were 97.8%-100.3%,with RSDs ranging from 0.4% to 1.2%.The method provides the benefits of simplicity,rapidity,sensitivity and accuracy,and therefore is applicable to the determination of gossypol in cotton seed kernel.

关 键 词：棉酚 棉籽仁 丙酮 高效液相色谱法(HPLC) 

分 类 号：TS207.3[轻工技术与工程—食品科学]