富硒双歧杆菌中硒的含量与形态研究  

Selenium Content and Speciation of Selenium-enriched Bifidobacterium

作　　者：梁佳[1,2] 李玉锋[1] 赵金璇[1,2] 王小燕[1,3] 李柏[1] 胡巍[2] 高愈希[1] 陈春英[1,4] 

机构地区：[1]中国科学院高能物理研究所,核分析技术重点实验室,纳米生物效应及安全性重点实验室,北京100049 [2]山东理工大学生命科学学院,山东淄博255049 [3]四川师范大学生命科学学院,四川成都610068 [4]国家纳米科学中心,北京100190

出　　处：《食品科学》2010年第5期121-124,共4页Food Science

基　　金：国家自然科学基金重大项目(10490180);欧盟第六框架计划项目(FOOD-CT-2006-016253);环境化学与生态毒理学国家重点实验室开放课题(KF2008-7)

摘　　要：利用电感耦合等离子质谱(ICP-MS)测定富硒双歧杆菌菌粉及其胶囊制品中硒的含量,研究富硒双歧杆菌细胞壁的破壁方法并利用高效液相色谱-电感耦合等离子质谱(HPLC-ICP-MS)对富硒双歧杆菌菌粉中硒的形态进行研究。结果表明,富硒双歧杆菌菌粉及其胶囊制品中硒的含量分别为(168.11±2.26)μg/g和(15.68±0.07)μg/g。与市售富硒产品相比较,富硒双歧杆菌胶囊中硒的含量是富硒大米的100倍,但不及富硒酵母产品中硒含量的1/10。富硒双歧杆菌产品中硒形态非常复杂,且含有许多不明硒化物,但其主要含硒氨基酸为硒代蛋氨酸,占总硒含量的(59.55±0.13)%。Selenium contents and speciations in powder and capsules containing selenium-enriched Bifidobacterium subjected to cell wall disruption by combinatorial ultrasonic and enzymatic hydrolysis method were determined by high performance liquid chromatography connected with inductively coupled plasma mass spectrometry （HPLC-ICP-MS）. Results indicated that the selenium contents in powder and capsules containing selenium-enriched Bifidobacterium were （168.11 ± 2.26） μg/g and （15.68 ± 0.07） μg/g, respectively. The selenium content in capsules containing selenium-enriched Bifidobacterium was nearly 100 times as high as that of commercially available selenium-enriched rice, but was less than one tenth of that of commercially available selenium-enriched yeast. A complicated selenium speciation was found in selenium-enriched Bifidobacterium products, and the major amino acid containing selenium was selenomethionine （SeMet）, which was up to 59% of total selenium amount.

关 键 词：富硒双歧杆菌 形态分析 高效液相色谱 电感耦合等离子质谱 

分 类 号：O657.72[理学—分析化学] Q814.9[理学—化学]