圆弧青霉PG37脂肪酶的生物信息学分析  

Bioinformatics Analysis of the Lipase from Penicillium cyclopium PG37

作　　者：李剑芳[1] 张慧敏[1] 邬敏辰 

机构地区：[1]江南大学食品学院,无锡214122 [2]江南大学医药学院,无锡214122

出　　处：《生物技术通报》2010年第3期191-195,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(20776061)

摘　　要：利用生物信息学软件对GenBank上登录的圆弧青霉PG37碱性脂肪酶(LipⅠ)进行预测和分析。结果表明,PG37LipⅠ全肽含多个疏水区域,无明显跨膜结构域,定位于胞外,N-末端20个氨基酸为信号肽;PG37LipⅠ成熟肽是等电点为6.16的疏水性稳定蛋白质,包含一个Lipase_3的结构域,属于α/β水解酶超家族,含磷酸化位点等多种功能性位点,具有脂肪酸代谢的功能;α-螺旋和不规则卷曲是其蛋白质二级结构的主要结构元件,在三级结构中,Ser132-Asp188-His2413个氨基酸残基组成酶活性中心。The sequence of alkaline lipase （LipⅠ ）from Penicillium cyclopium PG37, registered in GenBank, was analyzed by bioinfnrmatics tools. The results showed that PG37 LipⅠ complete peptide that locates in ecto-cell features many hydrophobic regions and a signal peptide, but without transmembrane domain. PG37 Lip I is stable, pl 6. 16, with strong hydrophobicity, and contain one Li- pase_3 domain and is classified into α/β hydrolase superfamily. There are many phosphorylation sites and other functional sites in the sequence which involved in fatty acid metabolism, α-helix and random coil are the main secondary structures. Ser^132-Asp^188-His^341 compose the enzyme active region in the tertiary structure.

关 键 词：生物信息学 圆弧青霉PG37 碱性脂肪酶 氨基酸序列 

分 类 号：Q814[生物学—生物工程]