AS1.398中性蛋白酶固定化条件的初步研究  

Study on Immobilization of AS1.398 Neutral Proteinase

作　　者：李泰明[1] 徐秀兰[1] 李伟 蒋企洲[1] 王旻[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生物制药学院,南京210009

出　　处：《药物生物技术》1998年第4期214-218,共5页Pharmaceutical Biotechnology

摘　　要：考察壳聚糖、卡拉胶、海藻酸钠、琼脂等固定化载体对 AS1 .398中性蛋白酶固定化的影响。确定 0 .3%戊二醛在 30℃ ,p H8.0的条件下处理壳聚糖 8～ 1 0 h,加酶液 ,固定 8h,酶活力回收约为 77% ,固定化酶最适作用温度为 55℃ ,最适作用 p H为 8.0 ;In this paper, we have examined the effect of immobilized support(Chitosan, Carrageenan, Sodium alginate, and Agar) on AS 1 398 neutral proteinase. The results showed that Chitosan was the best one among these supports. Three crosslinking agents (glutaraldehyde, chloroacetic acid and epichlorohydrin) were respectively used to immobilize AS 1 398 neutral proteinase. The activity recovery of immobilized enzyme reached 77% when glutaraldehyde 0 3%, pH8 0, treating time of chitosan 8～10h, enzymatic liquid 15ml(40mg/ml protein), immobilized time 8h. The optimum temperature and optimun pH of immobilized enzyme was 55℃ and8 0 respectively. We tested stability of immobilized enzyme on heating, alcohol and urea. Immobilized enzyme was superior to free enzyme in stability.

关 键 词：AS1.398 中性蛋白酶 固定化 载体 交联剂 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]