富硒米曲霉发酵罐放大实验的研究  

Study on scale-up of the fermenter of selenium-enriched aspergillus oryzae

作　　者：潘艳[1] 付璐[1] 刘斯淼[1] 陈亚光[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《中国调味品》2010年第3期51-54,共4页China Condiment

基　　金：富硒醋;酱油生产技术及工艺创新开发(20050418)

摘　　要：文章在富硒米曲霉摇瓶实验基础上进行了发酵罐放大实验。结果表明,分批发酵在第32 h胞内硒达到最大值2.896 mg/g,比摇瓶提高15%,此时菌体干重最大值9.8 g/L;指数补料在第30 h胞内硒最大值3.3 mg/g,比分批发酵提高14%,同时菌体干重最大值12.6 g/L,比分批发酵提高28%,指数补料更有利于菌体生长。The paper is about the scale-up experiment of Selenium-enriched Aspergillus Oryzae from shake flasks to fermenters.The results show that when Selenium-enriched Aspergillus Oryzae had been the content of selenium in a cell reached its maximum 2.896mg/g when it was cultivated for 32 hours,which had risen more 15% than in shake flask culture;the maximum of dry weight of Aspergillus Oryzae was 9.8g/L.And then,expotional Fed batch was made in accordance with parameters got from the batch fermentation.At the thirtieth hour of fermentation,the content of selenium reached its maximum： 3.3mg/L,which rose 14% as compared with that in the batch fermentation;the maximum of dry weight of Aspergillus Oryzae was 12.6g/L,which rose 28% from the batch fermentation.

关 键 词：富硒 米曲霉 发酵罐 

分 类 号：TS264.2[轻工技术与工程—发酵工程]