红芝组成型漆酶酶学性质的研究  

Enzymology Characteristics of Constitutive-Form Laccase Derived from Ganoderma lucidum

作　　者：赵亮亮[1,2] 谌斌[1,2] 李燕荣[3] 孙秋芳[1,2] 冯志菊[1,2] 

机构地区：[1]广西环境工程与保护评价重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《北方园艺》2010年第5期138-140,共3页Northern Horticulture

基　　金：广西环境工程与保护评价重点实验室基金资助项目(桂科能0702Z022);广西环境工程与保护评价重点实验室平台建设资助项目[桂教科研(2008)27号];广西研究生教育创新计划资助项目(2009106020830M66)

摘　　要：研究了温度、pH值、金属离子和抑制剂对红芝漆酶活性和稳定性的影响，测定该漆酶的Km值和Vmax。结果表明：红芝漆酶最适反应温度为60℃，低于50℃时较稳定；最适pH值为3．0，在pH2．5～4．0的范围表现出较强的稳定性；Cu^2＋、Mn^2＋、Mg^2＋对酶活有促进作用，而Cr^3＋、Hg^2＋、Fe^2＋和抑制剂EDTA、DMSO、SDS对酶活有抑制作用；以愈创木酚为底物时Km值为0．13mmol／L，最大反应速率Vmax为234nmol·mL^-1·min^-1。The effect of temperature, pH, inorganic ion and inhibitor on Ganoderzna lucidurn laccase activity and stability was studies. Meanwhile, the Km and Vrnax were measured. The result showed that the optimal temperature and optimal pH of laccase reaction were at 60℃ and pH 3.0, the laccase showed higher stability when temperature was not higher than 50℃, and the laccase was kept strongly active when pH ranged from 2. 5 to 4. 0. The laccase was activized by Cu^2＋ , Mn^2＋ and Mg^2＋ , and its activity was obviously inhibited by Cr^3＋, Hg^2＋ , Fe^2＋ and inhibitor. The Km and Vmax of laccase were 0. 13 mmol/L and 234 nmol·mL^-1·min^-1 respectively with guaiacol as substrate.

关 键 词：红芝 漆酶 组成型 酶学性质 

分 类 号：Q55[生物学—生物化学]