藁本提取物对2种柑桔贮藏病害病原菌的抑制作用研究  

ntifungal Activities of the extract from Ligusticum sinense against two Citrus Storage Pathogens

作　　者：贺磊[1,2] 胡军华[2] 姚廷山[2] 李鸿筠[2] 冉春[2] 刘浩强[2] 刘娟[1,2] 雷慧德[2] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]中国农业科学院柑桔研究所/国家柑桔工程技术研究中心

出　　处：《中国南方果树》2010年第2期6-10,共5页South China Fruits

基　　金：国家科技支撑计划(2006BAD22B03-B4);重庆市自然科学基金(CSTC,2008BB1272)资助

摘　　要：采用菌丝生长速率法,测定了藁本提取物对柑桔炭疽菌和柑桔绿霉菌的离体抑菌活性。结果表明,在藁本初提物浓度为3.500 mg/mL时,对绿霉病原菌抑制率为93.8%(培养3天)和85.4%(培养5天),对炭疽病菌抑制率为98.2%(培养3天)和91.71%(培养5天)。藁本提取物对炭疽菌的有效中浓度(EC50)分别为0.146 mg/mL(培养3天)和0.2109 mg/mL(培养5天);对柑桔绿霉菌的EC50为0.1544 mg/mL(培养3天)和0.3222 mg/mL(培养5天)。显微观察发现,藁本提取物处理后,2种病原菌菌丝出现扭曲、膨大、液泡增多、间隔改变及分枝增加等情况。藁本提取物抑菌活性的发现为进一步研究开发新型植物源农药提供了重要信息。The extract from Ligusticum sinense was investigated for its bioactivity against Penicillium digitatum and Colletotrichum gloeosporioides using mycelial growth rate method. The results showed that the inhibition rates were 93. 8% （3 d）and 85. 4% （5 d）against P. digitatum, and 98. 2%（3 d）and 91.7%（5 d）against C. gloeosporioides at 3. 5 mg/mL. Bioassay of extract showed that the ethanol extract was effective to P. digitatum and C. gloeosporioides, in which the EC40 was 0. 146 mg/mL（3 d）,0. 2109 mg/mL（5 d）and 0. 1544 mg/mL（3 d）, 0. 3222 mg/mL（5 d） respectively. Microscope observation showed that the mycelia of the two pathogens were abnormal, showing distorted, bulged, more-vacuolated, and more-branched. The results indicated that L. sinense is potentially a new botanic fungicide.

关 键 词：藁本提取物 柑桔绿霉病 柑桔炭疽病 抑菌活性 抑菌机理 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]