假单胞菌脂肪酶手性拆分研究进展  

Research progress in Pseudomonas lipase for chiral resolution

作　　者：季青春[1] 曹艳[1] 任伟[1] 何冰芳[1] 

机构地区：[1]南京工业大学生物与制药工程学院,材料化学工程国家重点实验室,江苏南京210009

出　　处：《化工进展》2010年第4期722-727,744,共7页Chemical Industry and Engineering Progress

基　　金：国家863计划(2007AA02Z210);国家973计划(2009CB724700)资助项目

摘　　要：脂肪酶是一种重要的工业用酶,本文从酶源角度综述了假单胞菌脂肪酶在拆分手性醇和酯等精细化学品中的应用,通过分子模拟解释其选择性并通过定向进化提高其选择性。由于假单胞菌脂肪酶天然耐有机溶剂的特性,可用于非水相生物催化。随着生物和化学领域的不断交叉,将进一步推动假单胞菌脂肪酶的开发和应用。Lipase is an important industrial enzyme.This review summarizes the application of Pseudomonas lipases in resolution of chiral alcohols and esters which are important intermediates for medical and fine chemicals.Besides,mechanism for the enantioselective performance of Pseudomonas lipase by molecular modeling,and the method for enantioselective enhancement by directed evolution are discussed.Furthermore,due to the nature of organic solvent tolerance,Pseudomonas lipases can be used in non-aqueous biocatalysis.With the interdisciplinary development of biology and chemistry,Pseudomonas lipases will have great potential in development and application in the future.

关 键 词：假单胞菌 脂肪酶 手性拆分 

分 类 号：Q814.9[生物学—生物工程] TQ463[化学工程—制药化工]