富n-3多不饱和脂肪酸猪油的生产及其对大鼠血脂的影响  

Production of Lard Enriched n-3 Polyunsaturated Fatty Acid and Its Effects on Serum Lipid in Rats

作　　者：郎婧[1] 石宝明[1] 张宏宇[1] 单安山[1] 高建[2] 郑兆利[2] 

机构地区：[1]东北农业大学动物营养研究所,哈尔滨150030 [2]黑龙江省兰西县种猪场,兰西151531

出　　处：《动物营养学报》2010年第2期410-415,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：黑龙江省科技攻关重大项目(GA07B201);黑龙江省博士后基金项目;东北农业大学创新团队和校人才基金资助

摘　　要：本试验旨在研究n-3多不饱和脂肪酸(PUFA)在猪腹脂中的富集,及富n-3 PUFA猪油对SD大鼠血脂水平的影响。选用平均体重(60±2)kg的育肥母猪(长白×东北民猪)15头,随机分成3组,每组5个重复,每个重复1头猪,单栏饲养。对照组饲喂玉米-豆粕-麦麸型基础日粮,试验组分别在基础饲粮基础上添加10%亚麻籽、10%亚麻籽+200mg/kg维生素E。56d后屠宰,取腹脂测定n-3 PUFA的含量。结果表明,试验组猪腹脂中n-3PUFA含量显著高于对照组(P<0.05)。选择60只36日龄SD大鼠,随机分为4组,每组3个重复,每个重复5只大鼠,对照Ⅰ组为不添加油脂的基础饲粮,对照Ⅱ组、试验Ⅲ组和试验Ⅳ组在基础饲粮中分别添加10%的上述试验各组获得的猪腹脂制成的液态油的饲粮,4周后测定各组大鼠血脂水平。结果表明:添加10%猪油各组大鼠血清总胆固醇含量显著高于不添加油脂组(P<0.05),对照Ⅱ组大鼠血清总胆固醇含量高于其他各试验组,但差异未达到显著水平(P>0.05)。各组大鼠血清甘油三酯和高密度脂蛋白胆固醇含量差异不显著(P>0.05)。对照Ⅱ组大鼠血清低密度脂蛋白胆固醇含量显著高于其他各组(P<0.05)。说明富含n-3多不饱和脂肪酸猪油可以起到降低血脂的作用,对预防心血管疾病具有一定作用。This experiment was conducted to study the enrichment of n-3 polyunsaturated fatty acids （n-3 PUFA） in abdominal fat of pigs, and the effects of lard enriched n-3 PUFA on serum lipid in rats. Fifteen gilts （Landrace × Min pig） with （60± 2） kg initial average weight were randomly allotted to three groups with five replicates and one gilt per replicate, which were reared in individual pens. The control diet was the corn-soybean meal-wheat bran diet, while the experimental diets were added 10% linseed and 10% linseed ＋ 200 mg/kg vitamin E based on the control diet, respectively. All pigs were slaughtered on 56 d, and sampled the abdominal fat to determine the content of n-3 PUFA. The results showed that n-3 PUFA content in lard of pigs in experiment groups was higher than that in control group （P〈0.05）. Sixty SD rats aged 36 d were assigned to four groups with three replicates, and five rats per replicate. Rats in control group Ⅰ were fed a basal diet without oil, and rats in control group Ⅱ , and experiment groups Ⅲ and Ⅳ were fed the diets which were added decocted lard from the corresponding groups of gilts, respectively. The content of serum lipid was determined after four weeks. The results showed that the rats fed 10% lard had significantly more total cholesterol in serum than those in the group without lard （P〈0. 05）, and total cholesterol in serum of rats in control group Ⅱ was higher than that in other groups, but the difference was not significant （P〉0. 05）. The triglyceride and high density lipoprotein cholesterol among groups were not significant （P〉0.05）. However, serum low density lipoprotein cholesterol of rats in control group Ⅱ was significantly higher than that in other groups （P〈0. 05）. It was indicated that the lard enriched in n-3 PUFA could decrease serum lipid, and play a role in preventing the formation of cardiovascular disease.

关 键 词：亚麻籽 n-3 PUFA 猪油 大鼠 血脂 

分 类 号：S816.48[农业科学—饲料科学]