紫菜中无机砷含量的分析研究  

Analysis and Study of the inorganic arsenic contents in dried purple seaweed

作　　者：吴成业[1] 钱卓真[1] 吴抒怀[2] 曹爱英 刘智禹[1] 刘淑集[1] 

机构地区：[1]福建省水产研究所,福建厦门361012 [2]厦门出入境检验检疫局,福建厦门361012 [3]北京市水产技术推广站,北京100021

出　　处：《福建水产》2010年第1期29-32,共4页Journal of Fujian Fisheries

基　　金：国家科技支撑计划(2006BAD05A18);福建省海洋与渔业局科技项目(闽海渔科06385)

摘　　要：本文针对紫菜中的无机砷超标问题,采用高效液相色谱—原子荧光光度法联用技术(HPLC-AFS)对紫菜中砷的形态进行分析,并测定了紫菜中的无机砷的含量。通过分析发现,紫菜中砷主要以一甲基胂酸、二甲基胂酸及砷糖、甜菜碱、砷胆碱等多种形态的有机砷化合物的形态存在于藻体中,无机砷的含量很少,仅在0.2mg/kg左右。采用HPLC-AFS能进行无机砷的分析测定时,由于三价砷的出峰时间与第1个峰的有机砷化合物出峰时间重叠,采用过氧化氢将其氧化成五价砷,可以很好地解决这一问题。通过验证,其加标回收率可达到80.19%,方法的准确性高,回收率符合检测的要求,适用于紫菜中无机砷的测定。To direct at the overproof of inorganic arsenic contents in dried purple seaweed, this paper investigates, by using the high efficiency liquid chromatography-Atom Fluoresence Method （HPLC-AFS） the form and detects the contents of inorganic arsenic in dried purple seaweed. We analyzed and found that there were variform organo-arsenie compounds in dried purple seaweed, such as methanearsenate, cacodylic acid and aresnosugars, lycine, arsenocholine, while a little inorganic arsenic, about 0. 2 mg/kg. Analyzing and detecting inorganic arsenic with HPLC-AFS, there was a time-interleaving between peak flowing out of trivalent arsenic and the first peak （ organo-arsenie compounds）, this problem could bo solved by oxidizing trivalent arsenic to quinquevalent arsenic. Finally, we validate that the standard addition recovery can reach 80. 19%, high precision and recovery can satisfy the detection requirements, which can be applied to the determination of the inorganic arsenic in dried purple seaweed.

关 键 词：紫菜 无机砷 砷形态 

分 类 号：O627.521[理学—有机化学]