Effects of levodopa on dopaminergic neurons and induced dyskinesia A radio-imaging study  

作　　者：Xiuying Cai Yan Kong Hongru Zhao Bin Zhang Chunfeng Liu 

机构地区：[1]Department of Neurology, First Hospital Affiliated to Soochow University, Suzhou 215006, Jiangsu Province, China [2]Department of Nuclear Medicine, First Hospital Affiliated to Soochow University, Suzhou 215006, Jiangsu Province, China [3]Department of Neurology, Second Hospitai Affiliated to Soochow University, Suzhou 215004, Jiangsu Province, China

出　　处：《Neural Regeneration Research》2010年第2期92-97,共6页中国神经再生研究（英文版）

基　　金：the Scientific Research Foundation Program of Ministry of Health,No.wkj.2005-2-030

摘　　要：BACKGROUND： Radio-imaging has been used in neurological diagnosis, in particular for extrapyramidal disease. Moreover, it has been extensively utilized for early diagnosis of Parkinson＇s disease （PD） patients and in animal studies. However, it has rarely been utilized to assess drug-induced side effects in PD. OBJECTIVE： To investigate changes in dopamine transporter expression in a rat model of PD through the use of radio-imaging taking ^99mTc-TRODAT-1 as an imaging agent, and to explore the effect of levodopa （L-dopa） on dopaminergic neurons and the possible mechanisms of dyskinesia induction. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Laboratory of Department of Nuclear Medicine, Soochow University from April 2006 to June 2007. MATERIALS： 6-hydroxydopamine was purchased from Sigma, USA and L-dopa was purchased from Shanghai Fuda Pharmaceutical, China. ^99mTcO4-fresh elutriant was provided by the Department of Nuclear Medicine, First Hospital Affiliated to Soochow University. TRODAT-1 image kit was provided by Jiangsu Atomic Energy Research Establishment, China. The SN-695B radioimmunoassay gamma counter was purchased from Shanghai Hesuo Rihuan Photoelectric Instrument, China. The AZ-CA256eZ-Scope portable y camera was purchased from Anzai Medical, Japan. METHODS： A total of 34 healthy, male, Sprague Dawley rats were selected. Thirty were used to establish a PD model by injecting 6-hydroxydopamine into the right medial forebrain bundle, and four were injected with normal saline and served as the sham-surgery group. At the end of 4 weeks, 21 successful PD models were selected and randomly assigned to the L-dopa （n = 15, 20 mg/kg per day）, model （n = 6, normal saline）, and sham-surgery （n = 4, no treatment） groups. After 1 month of treatment, involuntary movement was evaluated twice weekly in each rat. A total of 0.2 mL ^99mTc-TRODAT-1 was injected into the tail vein 2 days following drug termination, and images of dopamine transporters weBACKGROUND： Radio-imaging has been used in neurological diagnosis, in particular for extrapyramidal disease. Moreover, it has been extensively utilized for early diagnosis of Parkinson＇s disease （PD） patients and in animal studies. However, it has rarely been utilized to assess drug-induced side effects in PD. OBJECTIVE： To investigate changes in dopamine transporter expression in a rat model of PD through the use of radio-imaging taking ^99mTc-TRODAT-1 as an imaging agent, and to explore the effect of levodopa （L-dopa） on dopaminergic neurons and the possible mechanisms of dyskinesia induction. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Laboratory of Department of Nuclear Medicine, Soochow University from April 2006 to June 2007. MATERIALS： 6-hydroxydopamine was purchased from Sigma, USA and L-dopa was purchased from Shanghai Fuda Pharmaceutical, China. ^99mTcO4-fresh elutriant was provided by the Department of Nuclear Medicine, First Hospital Affiliated to Soochow University. TRODAT-1 image kit was provided by Jiangsu Atomic Energy Research Establishment, China. The SN-695B radioimmunoassay gamma counter was purchased from Shanghai Hesuo Rihuan Photoelectric Instrument, China. The AZ-CA256eZ-Scope portable y camera was purchased from Anzai Medical, Japan. METHODS： A total of 34 healthy, male, Sprague Dawley rats were selected. Thirty were used to establish a PD model by injecting 6-hydroxydopamine into the right medial forebrain bundle, and four were injected with normal saline and served as the sham-surgery group. At the end of 4 weeks, 21 successful PD models were selected and randomly assigned to the L-dopa （n = 15, 20 mg/kg per day）, model （n = 6, normal saline）, and sham-surgery （n = 4, no treatment） groups. After 1 month of treatment, involuntary movement was evaluated twice weekly in each rat. A total of 0.2 mL ^99mTc-TRODAT-1 was injected into the tail vein 2 days following drug termination, and images of dopamine transporters we

关 键 词：Parkinson＇s disease LEVODOPA dopamine transporter ^99MTC-TRODAT-1 NEUROIMAGING neural regeneration 

分 类 号：R[医药卫生]