肺癌MDM2蛋白表达与p53基因突变的关系  

THE CORRELATION BETWEEN MDM2 EXPRESSION AND p53 MUTATION IN HUMAN LUNG CANCER

作　　者：张骏[1] 郑杰[1] 方伟岗[1] 李红梅[1] 由江峰[1] 王洁良[1] 吴秉铨[1] 

机构地区：[1]北京医科大学病理学系

出　　处：《北京医科大学学报》1998年第6期488-490,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：研究肺癌ＭＤＭ２蛋白表达与ｐ５３突变之间的关系。方法：用免疫组化检测１２５例肺癌ｐ５３和ＭＤＭ２蛋白表达，以聚合酶链反应单链构象多态性（ＰＣＲＳＳＣＰ）方法检测ｐ５３基因第５～８外显子的突变。结果：ＰＣＲＳＳＣＰ检测阳性的５２例肺癌ＭＤＭ２阳性率为１９．２％；而阴性的７３例肺癌ＭＤＭ２蛋白阳性率为３５．６％。统计学检验显示肺癌ＭＤＭ２蛋白表达与ｐ５３突变之间呈负相关（χ２＝３．９８，Ｐ＜０．０５）；ＭＤＭ２蛋白阳性与肺癌患者的年龄、性别、肿瘤大小、组织学类型、分化程度、临床分期和ｐ５３蛋白表达无显著相关性。Objective: To study the correlation between MDM2 overexpression and p53 mutation in lung cancer. Methods: Immunohistochemistry and nonisotopic PCR SSCP were used. Results: The positive rate of MDM2 protein in 52 cases with SSCP positive was 19.2%, and in 73 cases with SSCP negative was 35.6%. In statistics, there was a negative correlation between MDM2 expression and p53 mutation (χ 2=3.98, P <0.05). The MDM2 expression status in human lung cancer was not associated with age, sex, clinical stage, histological subtypes, tumor sizes, lymphatic metastasis, and prognosis. Conclusion: MDM2 might play an important role in the development of the lung cancer without p53 mutations.

关 键 词：MDM2蛋白 肺肿瘤 P53基因 基因突变 

分 类 号：R734.2[医药卫生—肿瘤]