一株产木糖异构酶嗜热菌的分离鉴定及其培养条件优化  

Isolation,Identification of a Thermophilic Bacterium Producing Xylose Isomerase and Optimization of Its Culture Conditions

作　　者：殷燕[1] 张波[1] 

机构地区：[1]北京联合大学应用文理学院生物学系

出　　处：《食品与发酵工业》2010年第3期112-116,共5页Food and Fermentation Industries

摘　　要：从云南腾冲温泉水样中分离到1株产木糖异构酶活性较高的嗜热菌,根据菌株的形态特征和16SrD-NA序列,鉴定为Anoxybacillus flavithermus WL。采用单因素优化实验及正交设计,确定该菌株在木糖添加量15g/L、接种量3%、初始pH8.0、65℃的条件下发酵24h,达到最高酶活13.6U/mL。该菌株木糖异构酶粗酶液的最适反应温度为90℃。A thermophilic bacterium producing xylose isomerase was isolated from Yunnan Tengchong Hot Spring water samples. The strain was finally identified as Anoxybacillus flavithermus WL based on morphological and 16S rDNA gene sequence analysis. Its optimal fermentation conditions for xylose isomerase were determined as follows： cultivation temperature 65℃ , culture initial pH 8.0, inoculum density 3% and 15 g/L xylose for 24h. Under these conditions, enzyme activity reached 13.6 U/mL. Xylose isomerase from this strain performed the highest activity at 90℃.

关 键 词：ANOXYBACILLUS flavithermus WL D-木糖异构酶 正交设计 

分 类 号：TQ925[轻工技术与工程—发酵工程]