蚕蛹蛋白高效分解菌的分离筛选及部分酶学性质的研究  

Isolating and Screening of Effective Decomposing Strain of Silkworm Chrysalis Protein and Study on Parts of Its Enzymatic Properties

作　　者：孔军[1,2] 涂国全[1] 邝哲师[2] 赵祥杰[2] 叶明强[2] 罗国庆[2] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]广东省农业科学院农业生物技术研究所,广东广州510610

出　　处：《安徽农业科学》2010年第8期3911-3913,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省农业攻关计划项目(2007A0201000043);粤港关键领域重点突破招标项目(2006A25001002);国家现代农业产业技术体系建设专项资金资助项目

摘　　要：[目的]获得蚕蛹蛋白高效分解菌,探索其有关酶学性质。[方法]从腐败蚕蛹中采用稀释平板分离蛋白高效分解菌,经初筛和复筛后进行酶学性质试验,测定其酶活力,蚕蛹采用固体发酵后,测定其中小分子蛋白含量。[结果]获得1株较高效分解蚕蛹蛋白的细菌,其酶液作用最适pH值和温度分别为8.0和55℃,蚕蛹粉经该菌株发酵后中小分子蛋白含量高达15.09%,比未发酵的对照提高了95.72%。[结论]初步获得蚕蛹蛋白高效分解菌。[Objective]The research aimed to obtain an effectively-decomposing strain of silkworm chrysalis protein and discuss its enzymatic properties.[Method]The effectively-decomposing bacteria of protein was isolated from the decayed silkworm chrysalis by using dilution plate and its enzymatic properties were tested after primary screening and second screening.The enzyme activity was determined and the intermediate and small molecule protein content in silkworm chrysalis was measured after solid-state fermentation.[Result]An effectively-decomposing strain of silkworm chrysalis protein was obtained and its optimum pH value and temperature were 8.0 and 55 ℃ respectively.The content of intermediate and small molecule protein of silkworm chrysalis powder reached 15.09% after fermentation,95.72% higher than control without fermentation.[Conclusion]The effectively-decomposing bacteria of silkworm chrysalis protein was preliminarily obtained.

关 键 词：蚕蛹分解菌 分离筛选 中性蛋白酶 酶学性质 

分 类 号：Q939.97[生物学—微生物学]