补阳还五汤对大鼠胫前肌失神经肌萎缩的防治作用  

Preventive and therapeutic effect of buyang huanwu decoction on denervated tibial muscle atrophy of rats

作　　者：周岚[1] 梅晓云[1] 吴灏昕[1] 谢辉[1] 孙华林[2] 赵宗波[2] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046 [2]南通大学江苏省神经再生重点实验室,江苏南通226001

出　　处：《中国药理学通报》2010年第3期401-405,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No30873228)

摘　　要：目的研究补阳还五汤(BYHWD)对大鼠胫前肌失神经肌萎缩的防治作用。方法建立大鼠腓总神经夹伤模型,将造模后的60只♂SPF级SD大鼠随机分为6组:BYHWD高、中、低剂量组、弥可保组、模型组、假手术组,术后每日灌胃给药。术后18d,病理切片染色。形态学分析,计算胫前肌湿重比和横截面积。结果假手术组胫前肌横截面积较大,形态规则;模型组横截面积明显减小,结构紊乱,结缔组织明显增生;BYHWD各组与弥可保组横截面积较小,形态较规则,结缔组织增生不明显。与模型组相比,BYHWD各组胫前肌横截面积明显增加(P<0.01);BYHWD高剂量组胫前肌横截面积与弥可保组相比差异也有显著性(P<0.05)。与模型组相比,BYHWD高、中剂量组胫前肌湿重比明显增高(P<0.05或P<0.01)。BYHWD低、中、高组两两比较后,湿重比与横截面积差异均有显著性(P<0.05或P<0.01),表明BYHWD低、中、高剂量组湿重比与横截面积存在明显的剂量-效应关系。结论补阳还五汤对大鼠胫前肌失神经肌萎缩有明显的防治作用。Aim To study the preventive and therapeutic effects of Buyang Huanwu Decoction（BYHWD）on denervated tibial muscle atrophy of rats.Methods After 60 Sprague-Dawley rats were subjected to Common Peroneal nerve crush model of 5 mm injury,they were randomly divided into 6 groups for daily intragastric administration of drugs：BYHWD high-dose,medium-dose,low-dose groups,mecobalamin group（positive control）,model group,sham operation group.The drug administration lasted for 18 days.18 days after operation,slice,masson staining and analysis morphology were performed.The wet weight ratio and section area of tibial muscle were also measured.Results Tibial muscle section area of sham operation group was large,morphous was regular,while that of model group significantly diminished,structure was chaotic,and connective tissue hyperplasia was more obvious;Tibial muscle section area of BYHWD group and mecobalamin group was fairly diminished,morphous was fairly regular,and connective tissue hyperplasia was not obvious.Compared with model group,the section area of BYHWD group all increased significantly（P0.01）in a dose-dependent manner.Compared with mecobalamin group,the cross section area of BYHWD high-dose group was significantly larger（P0.05）.Compared with model group,the wet weight ratio of BYHWD high-dose and medium-dose group all increased significantly（P0.01 or P0.05）in a dose-dependent manner.Conclusion BYHWD has a significant effect of prevention and therapy on denervated tibial muscle atrophy of rats.

关 键 词：补阳还五汤 失神经 肌萎缩 大鼠 胫前肌 防治 中药 

分 类 号：R-332[医药卫生] R289.5