酪蛋白磷酸肽钙磷复合体对发酵乳杆菌生长影响的实验研究  

An experimental study of casein phosphopeptide-amorphic calcium phosphate on the growth of Lactobacillus fermentum

作　　者：刘兴容[1] 李艳萍[1] 徐皑[1] 

机构地区：[1]四川泸州医学院附属口腔医院,646000

出　　处：《实用口腔医学杂志》2010年第2期258-259,共2页Journal of Practical Stomatology

基　　金：四川泸州市科委科技计划项目资助(编号:01-14/343)

摘　　要：研究酪蛋白磷酸肽钙磷复合体(CPP-ACP)对发酵乳杆菌生长的影响,探讨CPP-ACP作为一种生物防龋制剂的可行性。将发酵乳杆菌接种到BHI培养基中厌氧培养,实验组中加入不同浓度〔0.5%～5.0%(W/V)〕的CPP-ACP溶液,厌氧培养48h后采用噻唑蓝(MTT)比色分析法测定细菌液的吸光度A值(λ=550nm)。随CPP-ACP浓度的升高,发酵乳杆菌的吸光度值降低,即发酵乳杆菌的活菌数减少(P<0.01)。CPP-ACP对发酵乳杆菌的生长具有抑制作用,且随CPP-ACP浓度的升高抑制作用增强。To investigate the effect of casein phosphopeptide-amorphic calcium phosphate （CPP-ACP） on the growth of Lactobacillus fermentum and to evaluate the possibility of CPP-ACP as a biological anticariogenic agent. Lactobacillus fermentum was inoculated in BHI with different concentrations of CPP-ACP and grew in anaerobic condition at 37℃ for 48 h. Used MTF method to evaluate the effects of CPPACP on the growth of Lactobacillus fermentum. CPP- ACP inhibited the growth of Laetobacillus fermentum effectively. With the concentration of CPP-ACP increasing, the values of A（550 rim） decreased. It was found that CPP-ACP had inhibitory effect on the growth of Lactobacillus fermentum, the inhibitory effect increased with CPP-ACP concentration.

关 键 词：酪蛋白磷酸肽钙磷复合体 发酵乳杆菌 生长 防龋 

分 类 号：R78[医药卫生—口腔医学]