苦参碱对大鼠原发性肝癌防治作用及其机制的研究  

Prevention and treatment of matrine on hepatic carcinoma of rat and its mechanism

作　　者：朱丹丹[1] 姚树坤[1] 闫建国[1] 潘琳[1] 李鸿[1] 

机构地区：[1]北京大学医学部中日友好医院消化内科,北京100028

出　　处：《中华肿瘤防治杂志》2010年第4期241-244,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30772859)

摘　　要：目的:研究苦参碱对二乙基亚硝胺(diethylnitrosamine,DEN)诱导的大鼠原发性肝癌的防治作用及其可能的机制。方法:SD大鼠46只,模型组:21只,DEN35mg/kg灌胃,2次/周,共16周;苦参碱组:15只,DEN灌胃的同时给予苦参碱3mg/(kg.d)灌胃,共20周;空白对照组:10只,每日等体积生理盐水灌胃。大鼠肝脏HE染色观察病理改变,免疫组化SP法观察凋亡抑制蛋白Survivin、细胞周期蛋白Cyclin B1在肝脏的表达。结果:苦参碱组较模型组死亡率降低(40.0%vs76.2%),P<0.05;病理检查示,苦参碱组较模型组成癌率低(53.3%vs90.5%),P<0.05;免疫组化检测示,Survivin和Cyclin B1两种蛋白在模型组表达多,空白组表达少,苦参碱组表达较空白组多而较模型组少,苦参碱组与模型组比较差异有统计学意义,P<0.05。结论:苦参碱对DEN诱导大鼠原发性肝癌有防治作用,其机制可能与降低Survivin及Cyclin B1表达有关。OBJECTIVE：To study the prevention and treatment of matrine on hepatic carcinoma of rat and its mechanism.METHODS：A total of 46 SD rats were divided into three groups randomly.A model group：21 rats were administered with diethylnitrosamine（DEN）35 mg/kd twice per week for 16 weeks to induce hepatic carcinoma;A matrine group：15 rats,except for the DEN,were administered with matrine 3 mg/（kg·d）for 20 weeks;A control group：10 rats were administered with the equal volume of 0.9% sodium chlorine every day.The mortality was caculated,the pathology of liver was investigated by H-E dyeing,and the expressions of Survivin and Cyclin B1 in the liver were assayed with immunohistochemistry in the three groups.RESULTS：The mortality of the matrine group was lower than that of the model group（40.0% vs 76.2%,P0.05）.The incidence of hepatic carcinoma in the matrine group was lower than that of the model group significantly（53.3% vs 90.5%,P0.05）.The expressions of Surviving and Cyclin B1 were high in the model group,nearly of no expression in the control group,and the expression of them were lower in the matrine group than those in the model group（P0.05）.CONCLUSION：Matrine can prevent and treat hepatic carcinoma of rat induced by DEN,and its mechanism may be to down-regulate the expressions of Survivin and Cyclin B1.

关 键 词：肝肿瘤 苦参碱 二乙基亚硝胺 SURVIVIN CYCLIN B1 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]