一株产细菌素乳酸菌的分离和鉴定  

Screening and identification of β-galactosidase-producing

作　　者：邓艳美[1] 

机构地区：[1]枣庄学院生命科学系,枣庄277106

出　　处：《食品科技》2010年第3期33-36,共4页Food Science and Technology

摘　　要：从市售3种酸乳中分离得到4株乳酸菌,通过发酵液的抑菌实验确定S1菌株为细菌素产生菌,综合S1菌株的形态学特征、生理生化特征将其初步鉴定为乳酸乳球菌(Lactococcus lactis.)。在排除过氧化氢和有机酸对指示菌的抑制作用后,确定该菌株产生的抑菌物质为细菌素,该细菌素对大肠杆菌具有较好抑制作用。This experiment isolated 4 different lactobacillus from three yogurts in the market and one can produce bacteriocin that named as S1.Phenotypic analyses including morphology and physiology charac teristics were carried out.By making a comprehensive view on all the results of taxonomy,the strain was identified as Lactococcus lactis.The supernatant of S1 could strongly inhibit indicator strains after excluded inhibitive effect of H2O2 and organic acid,which could be classed as bacteriocin.The bacteriocin which S1 produces has strong inhibitory effect to Escherichia coli,and the range of inhibitory is very different to Nisin.

关 键 词：细菌素 乳酸乳球菌 纯化 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]