不同栽培品种生大豆对大鼠内脏器官形态和肠道组织学的影响  

Effect of Different Cultivars Raw Soybean on the Internal Organ Morphology and Gut Histology in Rats

作　　者：潘洪彬[1,2] 谷春梅[1] 孙泽威[1] 秦贵信[1] 

机构地区：[1]吉林农业大学动物科技学院,长春131108 [2]云南农业大学云南省动物营养与饲料重点实验室,昆明650201

出　　处：《中国农学通报》2010年第8期17-20,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"大豆凝集素在不同种属动物体内消化动力学及抗营养作用比较研究"(30471251)

摘　　要：为比较不同栽培品种生大豆中抗营养因子对大鼠内脏器官形态和肠道组织学影响,选用(50±2)日龄的Wister大鼠60只,随机平均分6组,分别饲喂以吉林小粒6、吉林30、吉林45、吉农7、丰交7607栽培品种生大豆和膨化大豆粕(对照组)为主要蛋白源的日粮,30天解剖大鼠,内脏器官称重,肠道测量长度、制作组织切片。结果表明:吉农7日粮组大鼠胰腺重量明显低于吉林小粒6、吉林30和丰交7607日粮组大鼠(P<0.05);吉林小粒6日粮组大鼠小肠长度、小肠重量明显高于对照组大鼠(P<0.05);吉林45、吉农7日粮组大鼠的空肠绒毛长度;空肠隐窝深度明显高于吉林小粒6、吉林30、丰交7607日粮组(P<0.05)。各品种生大豆日粮对大鼠抗营养影响为吉林小粒6>吉林30>丰交7607>吉林45>吉农7。The objective of this study was to investigate the antinutritional effect of five different China raw soy- bean cultivars on the internal organ morphology and gut histology in rats.Sixty rats aged 50±2 days were randomLy divided into six groups, with each group involved 10 replicates. Test groups were fed on the diets containing raw soybean cultivars： Jinlinxiaoli6, Jilin30, Jilin45,Jinong7, and fengjiao7607, respectively. Control groupdiets containing extruded full-fat soybean （EFFS）. The whole experiment duration was 30 days, All rats were slaughtered on 30 d. pancreas＇ liver, speen, kidney and stomach were collected quickly for weighting, duodenum central region and jejunuml/3 and ileum 1/2 were collected to making histological section for light microscope to inspected, respectively. Then, the gut measured length and weight. Results showed： The pancreas weight of rats fed on the diets containingfinong7 cutivars raw soybean was significantly lower compared to containingfilinxiaoli6, filin30JCengjiao7607 groups （P 〈 0.05）, the small intestine length and height of rats fed on the diets containingfilinxiaoli6 cutivars raw soybean were significantly heighter compared to the control group （P 〈 0.05）, the jejunum villus height： the jejunum crypt depth of rats fed on the diets containingfilin45,jinong7 cutivars raw soybean were significantly difference compared to the filinxiaoli6,jilin30, fengfiao7607 groups（P 〈 0.05）. Effect of antinutritional in feeding rats with raw sobean diets is Jinlinxiaoli6 〉 Jilin30 〉fengjiao7607〉 Jilin45 〉 Jinong7.

关 键 词：抗营养 生大豆 品种 大鼠 

分 类 号：S816.42[农业科学—饲料科学]