响应面分析法优化毛竹叶挥发油提取工艺  

Response Surface Methodology as An Approach to Optimization of Extraction of Volatile Oil from Leaves of Phyllostachys heterocycla(Carr.) Mitford cv.Pubescens Mazel ex H.de leh

作　　者：秦娇[1] 姚永红[1] 侯智霞[2] 张柏林[1] 吕兆林[3] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学教育部省部共建森林培育与保护重点实验室,北京100083 [3]北京林业大学分析测试中心,北京100083

出　　处：《食品科学》2010年第6期1-5,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD19B08);国家自然科学基金项目(30500349);北京市自然科学基金项目(6062020)

摘　　要：以毛竹叶为材料,利用改造后的挥发油提取装置,提取竹叶挥发油。在单因素试验的基础上,利用Box-Benhnken中心组合试验和响应面分析法,确定了提取竹叶挥发油的最适工艺条件,即平均粒径为10目的竹叶粉在水中加热回流、液固比35:1(mL/g)、提取时间4.5h,竹叶挥发油实际得率为0.32%,预测值为0.35%。与传统方法相比,该方法具有操作简便、挥发油得率高、挥发油中化合物信息丰富的特点。Phyllostachys heterocycla（Carr.） Mitford cv.Pubescens Mazel ex H.de leh.leaves were used as raw material to extract volatile oil using a modified oil extraction apparatus.Based on single factor experiments,the optimal extraction process was determined using central composite design combined with response surface methodology.Results showed that the optimal extraction process was based on heat reflux extraction of powdered raw material below 10 mesh in particle size with distilled water at a liquid/solid ratio of 35：1（mL/g） for 4.5 h.The observed and predicted values of extraction yield of volatile oil from Phyllostachys heterocycla（Carr.） Mitford cv.Pubescens Mazel ex H.de leh.leaves were 0.32% and 0.35%,respectively.This method has some advantages over conventional method,like simplicity as well as high extraction yield and abundant chemical composition of volatile oil.

关 键 词：毛竹叶 挥发油 提取 响应面分析 

分 类 号：TS201.22[轻工技术与工程—食品科学]