响应面法优化天冬多糖的提取条件  

Optimization of Extraction of Radix Asparagi Polysaccharides Using Response Surface Methodology

作　　者：王明艳[1] 鲁加峰[1] 王晓顺[1] 许兴友[1] 

机构地区：[1]淮海工学院江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《食品科学》2010年第6期91-95,共5页Food Science

基　　金：江苏省海洋资源开发研究院科技开放基金项目(JSIMR09B05);淮海工学院自然基金项目(Z2008021)

摘　　要：以天冬多糖提取率(Y1)和含量(Y2)为响应值,利用响应面法对天冬多糖提取工艺进行多目标同步优化。根据统计模型发现3个影响因素——提取时间、提取温度、液料比均对多糖提取率和含量有显著影响。由响应面三维及等高线叠加图推测得到天冬多糖提取率与含量均较高的最佳提取工艺参数为提取时间4.2h,提取温度78℃,液料比22:1(mL/g)。验证优化工艺参数得到多糖提取率达到5.24%,多糖含量达到26.42%,与模型预测值非常接近。采用响应面法对天冬多糖提取条件进行优化合理可行。Optimal hot water extraction of polysaccharides from Radix Asparagi was explored using central composite design（CCD） combined with response surface methodology（RSM）.CCD experimental data were fitted to create two mathematical models describing yield（Y1） and purity（Y2） of Radix Asparagi polysaccharides（RAP） obtained at different levels of critical parameters,such as extraction temperature and time and material/liquid ratio.Results showed that the above parameters all had a remarkable effect on Y1 and Y2,and extraction for 4.2 h at 78 ℃ with a material/liquid ratio of 22：1（mL/g） resulted in an optimal compromise between Y1 and Y2.The observed values of Y1 and Y2 were 5.24 % and 26.42%,respectively,exhibiting a good agreement with their predicted values.This reveals the good applicability and feasibility of RSM for optimizing extraction process of polysaccharides from Radix Asparagi.

关 键 词：天冬多糖 提取 响应面法 重叠等高线图 

分 类 号：R282.71[医药卫生—中药学]