海产品中沙门氏菌的分离及其ERIC-PCR指纹分析  

Use of ERIC-PCR to identify and analyze the DNA fingerprints of Salmonella in seafood

作　　者：李晓霞[1] 于爱莲[1] 邱玉玉[2] 张海涛[3] 于广福[1] 陈秀春[1] 庄东明[1] 

机构地区：[1]泰山医学院基础部病原学教研室,山东泰安271000 [2]泰山医学院生物科学系,山东泰安271000 [3]泰安市妇幼保健医院,山东泰安271000

出　　处：《中国病原生物学杂志》2010年第3期164-167,共4页Journal of Pathogen Biology

摘　　要：目的了解常见市售鲜活海产品沙门氏菌污染情况及不同菌株间遗传相似性,为沙门氏菌的流行病学研究提供有价值的信息。方法采集市售常见海鱼、贝类和蟹等海产品,按照常规方法分离沙门氏菌,分析分离菌的ERIC-PCR DNA指纹图谱。结果共检查191份样品,从42份中分离到沙门氏菌45株。ERIC-PCR DNA指纹图谱分析,45株分离菌被分为两个大的分支,遗传相似性在50%~100%之间。结论市售海产品有沙门菌污染,同一种海产品污染菌的基因型可有不同,不同海产品之间也存在相同基因型沙门氏菌株污染。Objective To determine the prevalence of Salmonella infection in fresh seafood and genetic similarities between different strains and to then provide valuable reference information with regard to Salmonella epidemiology.Methods Fresh fish,shellfish,and crab samples were obtained from local markets.A conventional culture-based study was performed and Salmonella strains were isolated and identified.Salmonella strains were amplified using ERIC-PCR and then their DNA fingerprints were analyzed.Results Salmonella was detected in 42/191 samples and 45 Salmonella strains were obtained.Forty-five Salmonella strains were divided into two important branches and the genetic similarity index was 50%-100%.Conclusion Seafood was contaminated with different strains of Salmonella.Different genotypes of Salmonella were present in the same seafood sample and the same genotype was present in different seafood samples.

关 键 词：海产品 沙门氏菌 分离鉴定 细菌基因间重复共有序列PCR(ERIC-PCR) 

分 类 号：R378.22[医药卫生—病原生物学]