β-环状糊精葡萄糖基转移酶菌株的双重诱变育种  

Double-mutation breeding of strain producing β-cyclodextrin glycosyltransferase

作　　者：何飞燕[1] 廖威[1] 莫于旺[1] 林华[1] 

机构地区：[1]广西职业技术学院食品技术系,广西南宁530226

出　　处：《中国酿造》2010年第4期154-157,共4页China Brewing

基　　金：广西科技厅基金项目(桂科自0833163)

摘　　要：建立以酚酞作指示剂,在琼脂固态培养基上筛选环糊精糖基转移酶高产突变株的平板快速筛选法,从土壤分离物中筛选到1株葡萄糖基转移酶(CGTase)产生菌gxmf1,于37℃、250r/min摇床发酵3d,产酶1524U/mL。该菌株经紫外线和LiCl诱变处理后,筛选得到突变株B15,产环糊精糖基转移酶达5416U/mL,较出发菌株提高255%。连续5代传代试验表明突变株B15产酶基本稳定。The method of fast screening high-production of β-cyclodextrin glycosyltransferase strain on agar solid medium containing soluble starch was established using the phenolphthalein as an indicator. A strain gxmfl selected from soil was found to produce 1524 U/ml eyclodextrin glyeosyltransferase （CGTase）after 3 d fermentation at 37℃ under 250 r/min. A mutant B 15 was obtained by double mutation of ultraviolet radiation and LiCI sulfate. The activity of the strain was enhanced by 255% （5416 U/ml）. The production of cyclodextrin glyeosyltransferase by mutant BI5 was stable after 5 generations culture.

关 键 词：育种 环糊精糖基转移酶 双重诱变 

分 类 号：Q93-331[生物学—微生物学]