甘露醇添加剂对毛细管无胶筛分电泳分离DNA的影响  

Influence of Mannitol Additive on DNA Separation by Capillary Non Gel Sieving Electrophoresis

作　　者：韩富天[1] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所

出　　处：《色谱》1998年第6期489-491,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金

摘　　要：在纤维素衍生物筛分体系中加入甘露醇添加剂大大提高了分离能力，在较低筛分剂浓度条件下可得到满意的分离。同时还对甘露醇影响分离的机理做出了解释。Polymer solutions with high concentration were employed as sieving buffer in order to obtain satisfactory separation of smaller DNA fragments with length of less than 1 000 base pairs. The concentrated polymer solution had very high viscosity, which was diffcult to be pumped into or out of the capillary. Mannitol additive can enhance the sieving ability of hydroxy propylmethylcellulose (HPMC) solution in a Tris Borate EDTA background electrolyte. With the existence of mannitol, good separation of PGEM 3Zf(+)/HaeⅢ was able to be obtained in a less concentrated HPMC solution with relatively low viscosity. The optimum mass concentration of mannitol was investigated and it was found that 60 g/L mannitol could give the best results. Mannitol chain could be formed through hydrogen bond among mannitol, HPMC and borate. This kind of mannitol chain shaped the network and decreased the pore size, so the separation was greatly enhanced.

关 键 词：毛细管电泳 DNA片段 甘露醇 分离 HPMC 无胶筛分 

分 类 号：Q523.03[生物学—生物化学]