转萝卜过氧化物酶基因毕赤酵母株系培养条件的优化  

The Optimum Cultivation for Improved Heterologous Expression of Radish Peroxidase in Pichia pastoris

作　　者：王一帆[1] 李芳军[1] 杨静[1] 王丽[1] 王林嵩[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《生物技术通报》2010年第4期203-206,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30570135);国家大学生创新性实验计划项目(081047609);河南师范大学大学生创新性实验计划项目(2008405);河南师范大学(国家级)实验教学示范中心大学生创新性实验计划项目

摘　　要：为了提高转萝卜过氧化物酶基因RsPrx1毕赤酵母株系的表达产量,对培养条件进行优化。试验结果表明,26℃,pH7.4,甲醇浓度0.5%,通气面积1.5cm2,225r/min条件下,萝卜过氧化物酶RsPrx1表达量达50.95U/mL。在优化条件下,对培养基补充方式和菌株的遗传稳定性研究表明,彻底更换培养基较补充培养基提高3倍得率,转基因菌株遗传稳定性较高。The optimum cultivation have been investigated for improved heterologous expression of RsPrx1（a peroxidase of radish）in Pichia pastoris.The results showed that the expression of RsPrx1 could reached 50.95 U/mL in 26℃,pH7.4,0.5% methanol,1.5 cm^2 aeration area,225 r/min.In the optimum cultivation,the total activity of peroxidase was about 3 times higher in absolutely replaced medium continuous culture than that partly added medium continuous culture.The PCR results showed the transgenic strain was hereditary stability.

关 键 词：萝卜过氧化物酶 毕赤酵母 转基因 培养条件 

分 类 号：TQ925[轻工技术与工程—发酵工程]