谷氨酸脱羧酶在新型羧甲基化交联烯丙基葡聚糖凝胶树脂上的固定化  

IMMOBILIZATION OF L-GLUTAMIC DECARBOXYLASE ON CARBOXYMETHYL DEXTRAN RESIN

作　　者：吴国琪[1] 凌达仁[1] 王忱[1] 王钒[1] 宋国强[1] 

机构地区：[1]江苏石油化工学院应用化学系,常州213016

出　　处：《离子交换与吸附》1998年第6期526-532,共7页Ion Exchange and Adsorption

基　　金：江苏省自然科学基金

摘　　要：本文首次用合成的羧甲基化的以N，N’-甲叉双丙烯酰胺交联的烯丙基葡聚糖（简称CM—CADB）凝胶生化树脂为新型载体，研究了谷氨酸脱羧酸在CM—CADB树脂上的固定化与环境的依赖关系。确定了酶固定化最佳条件：缓冲体系为pH4．4，0．10mol／L磷酸氢二钠-0．05mol／L柠檬酸，载体为交联度35％，交换容量3．0meq／g的CM－CADB凝胶树脂，吸附平衡时间4h以上，酶用量为30．0mg／gdryresin，固定化环境温度控制在40℃。从理论上分析了上述结果。The immobilization of L-glutamic decarboxylase (GDC) on the CM-CADB (carboxymethyl-copolymer of allyl dextran and N, N'-methylene-bisacrylamide) ion exchange resin has been studied in this paper. The correlation ofimmobilization with the surroundings was researched. The conditions for the immobilization of GDC were optimized by studying the buffer solutions and the various parameters: adsorption equilibrium time. amount of the enzyme, PH, temperature, ionic strength and ion exchange capacity of CM -- CADB resin. The results were discussed.

关 键 词：谷氨酸脱羧酶 分析 固定化酶 羧甲基化葡聚糖 

分 类 号：TS202[轻工技术与工程—食品科学]